Cloning and molecular characterization of the Chinese hamster ERCC2 nucleotide excision repair gene
Abstract
The Chinese hamster ERCC2 nucleotide excision repair gene, encoding a presumed ATP-dependent DNA helicase, was cloned from the V79 cell line, and its nucleotide sequence was determined. The {approximately}15-kb gene comprises 23 exons with a 2283-base open reading frame. The predicted 760-amino-acid protein is 98% identical to the human ERCC2/EXP (760 amino acids), 51% identical to the Saccharomyces cerevisiae RAD3 (778 amino acids), and 54% identical to the Schizosaccharomyces pombe rad15 (772 amino acids) proteins. The promoter region of the hamster ERCC2 gene contains a pyrimidine-rich stretch (42 nucleotides, 88% C+T) similar to sequences found in the promoter regions of two other nucleotide excision repair genes, a GC box, a putative {alpha}-Pal transcription factor binding site, and two CAAT boxes. There is no apparent TAATA box. No consensus polyadenylation sequence (AATAAA or its variants) was found with 663 bases 3{prime} of the translation termination codon. 54 refs., 2 figs., 2 tabs.
- Authors:
-
- Lawrence Livermore National Lab., CA (United States); and others
- Publication Date:
- OSTI Identifier:
- 183695
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Journal Article
- Journal Name:
- Genomics
- Additional Journal Information:
- Journal Volume: 23; Journal Issue: 3; Other Information: PBD: Oct 1994
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; DNA-CLONING; DNA SEQUENCING; TRANSCRIPTION; PROTEINS; CHO CELLS; DNA REPAIR; DNA HELICASES; NUCLEOTIDES; SACCHAROMYCES CEREVISIAE; EXONS; AMINO ACIDS; PYRIMIDINES; TRANSCRIPTION FACTORS; CODONS; PROBES; DNA HYBRIDIZATION
Citation Formats
Kirchner, J M, Salazar, E P, and Lamerdin, J E. Cloning and molecular characterization of the Chinese hamster ERCC2 nucleotide excision repair gene. United States: N. p., 1994.
Web. doi:10.1006/geno.1994.1547.
Kirchner, J M, Salazar, E P, & Lamerdin, J E. Cloning and molecular characterization of the Chinese hamster ERCC2 nucleotide excision repair gene. United States. https://doi.org/10.1006/geno.1994.1547
Kirchner, J M, Salazar, E P, and Lamerdin, J E. 1994.
"Cloning and molecular characterization of the Chinese hamster ERCC2 nucleotide excision repair gene". United States. https://doi.org/10.1006/geno.1994.1547.
@article{osti_183695,
title = {Cloning and molecular characterization of the Chinese hamster ERCC2 nucleotide excision repair gene},
author = {Kirchner, J M and Salazar, E P and Lamerdin, J E},
abstractNote = {The Chinese hamster ERCC2 nucleotide excision repair gene, encoding a presumed ATP-dependent DNA helicase, was cloned from the V79 cell line, and its nucleotide sequence was determined. The {approximately}15-kb gene comprises 23 exons with a 2283-base open reading frame. The predicted 760-amino-acid protein is 98% identical to the human ERCC2/EXP (760 amino acids), 51% identical to the Saccharomyces cerevisiae RAD3 (778 amino acids), and 54% identical to the Schizosaccharomyces pombe rad15 (772 amino acids) proteins. The promoter region of the hamster ERCC2 gene contains a pyrimidine-rich stretch (42 nucleotides, 88% C+T) similar to sequences found in the promoter regions of two other nucleotide excision repair genes, a GC box, a putative {alpha}-Pal transcription factor binding site, and two CAAT boxes. There is no apparent TAATA box. No consensus polyadenylation sequence (AATAAA or its variants) was found with 663 bases 3{prime} of the translation termination codon. 54 refs., 2 figs., 2 tabs.},
doi = {10.1006/geno.1994.1547},
url = {https://www.osti.gov/biblio/183695},
journal = {Genomics},
number = 3,
volume = 23,
place = {United States},
year = {Sat Oct 01 00:00:00 EDT 1994},
month = {Sat Oct 01 00:00:00 EDT 1994}
}