Method for construction of normalized cDNA libraries

Patent · Tue Jan 09 00:00:00 EST 1996

OSTI ID:170463

Soares, M B; Efstratiadis, A

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form. The method comprises: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

• Research Organization: Columbia Univ., New York, NY (United States)
• DOE Contract Number: FG02-91ER61233
• Assignee: Columbia Univ., New York, NY (United States)
• Patent Number(s): US 5,482,845/A/
• Application Number: PAN: 8-126,594
• OSTI ID: 170463
• Resource Relation: Other Information: PBD: 9 Jan 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA
GENETIC MAPPING
MOLECULAR STRUCTURE
BIOCHEMISTRY
MOLECULAR BIOLOGY