Detection of mercury compounds using invertase-glucose oxidase-based biosensor

Book · Sun Dec 31 00:00:00 EST 1995

OSTI ID:153282

Amine, A ^[1]; Cremisini, C ^[2]; Palleschi, G ^[3]

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1. Faculte des Sciences et Techniques, Mohammadia (Morocco). Dept. Biologie
2. ENEA, Rome (Italy). Dipartimento Ambiente
3. Univ. di Napoli Federico II, Portici (Italy). Dipartimento di Scienze

Mercury compounds have been determined with an electrochemical biosensor based on invertase inhibition. When invertase is in presence of mercury its activity decreases; this causes a decrease of glucose production which is monitored by the glucose sensor and correlated to the concentration of mercury in solution. Parameters as pH, enzyme concentration, substrate concentration, and reaction and incubation time were optimized. Mercury compounds determination using soluble or immobilized invertase were reported. Results showed that the inhibition was competitive and reversible. Mercury compounds can be detected directly in aqueous solution in the range 2--10 ppb.

• OSTI ID: 153282
• Report Number(s): CONF-9506232-; ISBN 0-8194-1862-5; TRN: IM9602%%218
• Resource Relation: Conference: Society of Photo-optical Instrumentation Engineers (SPIE) conference, Munich (Germany), 19-23 Jun 1995; Other Information: PBD: 1995; Related Information: Is Part Of Environmental monitoring and hazardous waste site remediation; Vo-Dinh, T. [ed.] [Oak Ridge National Lab., TN (United States)]; Niessner, R. [ed.] [Technische Univ. Muenchen (Germany)]; PB: 613 p.; SPIE Proceedings Volume 2504
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
MERCURY
BIOASSAY
ISOMERASES
ENZYME ACTIVITY
INHIBITION
EXPERIMENTAL DATA
ELECTROCHEMISTRY
MEASURING METHODS