Distribution of TCDD in blood constituents of rats and humans
- Univ. of Kansas Medical Center, Kansas City, KS (United States). Dept. of Pharmacology, Toxicology and Therapeutics
- GSF-Institut fuer Toxikologie, Neuherberg (Germany). Section of Environmental Toxicology
- GSF-Institut fuer Oekologische Chemie, Neuherberg (Germany)
Whole blood or plasma from male human volunteers or pooled from male Sprague-Dawley rats was incubated with varying amounts of {sup 3}H-2,3,7,8-tetrachlorodibenzo-p-dioxin ({sup 3}H-TCDD). Blood was separated into cellular, protein and lipoprotein fractions by centrifugation. The distribution of {sup 3}H-TCDD between lipoproteins and plasma proteins was independent of {sup 3}H-TCDD concentration in the range of 65 fmol--1 nmol/ml plasma. The distribution of {sup 3}H-TCDD between the various lipoprotein fractions depended only on their relative content of total cholesterol + triglycerides. The partitioning of {sup 3}H-TCDD between lipoproteins and plasma proteins was inversely proportional, whereas the distribution between the cellular fraction and the lipoproteins was directly proportional to the total plasma cholesterol + triglyceride content. As a consequence of species differences in blood composition, the major part of {sup 3}H-TCDD-associate radioactivity was recovered from lipoproteins in human blood but from erythrocytes in rat blood. A mathematical description of the distribution of TCDD between blood components is presented.
- OSTI ID:
- 153260
- Report Number(s):
- CONF-9506232-; ISBN 0-8194-1862-5; TRN: IM9602%%215
- Resource Relation:
- Conference: Society of Photo-optical Instrumentation Engineers (SPIE) conference, Munich (Germany), 19-23 Jun 1995; Other Information: PBD: 1995; Related Information: Is Part Of Environmental monitoring and hazardous waste site remediation; Vo-Dinh, T. [ed.] [Oak Ridge National Lab., TN (United States)]; Niessner, R. [ed.] [Technische Univ. Muenchen (Germany)]; PB: 613 p.; SPIE Proceedings Volume 2504
- Country of Publication:
- United States
- Language:
- English
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