Identification of Proteins in Human Cytomegalovirus (HCMV) Particles: the HCMV Proteome

Journal Article · Fri Oct 15 00:00:00 EDT 2004 · Journal of Virology, 78(20):10960-10966

DOI:https://doi.org/10.1128/JVI.78.23.13395.2004· OSTI ID:15010751

Varnum, Susan M; Streblow, Daniel N; Monroe, Matthew E; Smith, Patricia; Auberry, Kenneth J; Pasa-Tolic, Liljiana; Wang, Dai; Camp, David G; Rodland, Karin D; Wiley, H S; Britt, William; Shenk, Thomas; Smith, Richard D; Nelson, Jay

Human cytomegalovirus (HCMV), a member of the herpes virus family, is a large complex enveloped virus composed of both viral and cellular gene products. While the sequence of the HCMV genome has been known for over a decade, the full set of viral and cellular proteins that compose the HCMV virion are unknown. To approach this problem we have utilized gel-free two-dimensional capillary liquid chromatography-tandem mass spectrometry (MS/MS) and Fourier transform ion cyclotron resonance MS to identify and determine the relative abundances of viral and cellular proteins in purified HCMV AD169 virions and dense bodies. Analysis of the proteins from purified HCMV virion preparations has indicated that the particle contains significantly more viral proteins than previously known. In this study, we identified 71 HCMV-encoded proteins that included 12 proteins encoded by known viral open reading frames (ORFs) previously not associated with virions and 12 proteins from novel viral ORFs. Analysis of the relative abundance of HCMV proteins indicated that the predominant virion protein was the pp65 tegument protein and that gM rather than gB was the most abundant glycoprotein. We have also identified over 70 host cellular proteins in HCMV virions, which include cellular structural proteins, enzymes, and chaperones. In addition, analysis of HCMV dense bodies indicated that these viral particles are composed of 29 viral proteins with a reduced quantity of cellular proteins in comparison to HCMV virions. This study provides the first comprehensive quantitative analysis of the viral and cellular proteins that compose infectious particles of a large complex virus.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 15010751
• Report Number(s): PNNL-SA-43398; 400412000
• Journal Information: Journal of Virology, 78(20):10960-10966, Journal Name: Journal of Virology, 78(20):10960-10966
• Country of Publication: United States
• Language: English

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