Erratum to: Automated Sample Preparation Method for Suspension Arrays using Renewable Surface Separations with Multiplexed Flow Cytometry Fluorescence Detection

Journal Article · Thu Apr 10 00:00:00 EDT 2003 · Analytica Chimica Acta

DOI:https://doi.org/10.1016/S0003-2670(03)00198-3· OSTI ID:15003616

Grate, Jay W; Bruckner-Lea, Cindy J; Jarrell, Ann E; Chandler, Darrell P

In this paper we describe a new method of automated sample preparation for multiplexed biological analysis systems that use flow cytometry fluorescence detection. In this approach, color-encoded microspheres derivatized to capture particular biomolecules are temporarily trapped in a renewable surface separation column to enable perfusion with sample and reagents prior to delivery to the detector. This method provides for separation of the biomolecules of interest from other sample matrix components as well as from labeling solutions.

• Research Organization: Pacific Northwest National Lab., Richland, WA (US), Environmental Molecular Sciences Laboratory (US)
• Sponsoring Organization: US Department of Energy (US)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 15003616
• Report Number(s): PNNL-SA-37670; ACACAM; 3450; NN2004000; TRN: US201015%%1
• Journal Information: Analytica Chimica Acta, Vol. 482, Issue 1; Other Information: PBD: 10 Apr 2003; ISSN 0003-2670
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
FLUORESCENCE
CELL FLOW SYSTEMS
MICROSPHERES
SAMPLE PREPARATION
BIOLOGICAL MATERIALS
SEPARATION PROCESSES
AUTOMATION
SUSPENSIONS
ENVIRONMENTAL MOLECULAR SCIENCES LABORATORY
ADDED ERRATUM TO: TO TITLE KAB 4/29/03