Cellulase assays: Methods from empirical mathematical models
- NREL, Golden, CO (United States)
Recent advances in the assay of cellulose preparations by Sattler et al. [Biotech. Bioeng. 33, 1221-1234 (1989)] describe a relationship between extent of hydrolysis, reaction time, and enzyme concentration. This procedure permits the ranking of the effectiveness of different enyzmes and of different pretreatment methods. For this method, cellulose hydrolysis data collected from hyperbolic functions of substrate concentration versus cellulose enzyme concentration at various timed incubations are collected. A double reciprocal plot based on the relationship (Y/C{sub 6}){sup {minus}1}=(KC/{sub 0}/Y{sub max})[E]{sup {minus}1} + (Y{sub max}/C{sub 0}){sup {minus}1} where Y/C{sub 0} is the fraction of substrate hydrolyzed; [E] is given in FPU/g substrate initially added; and Y{sub max}/C{sub 0} is the where Y/C{sub 0} is the fraction of substrate hydrolyzed an an infinite enzyme concentration. The y-axis intercept in the double reciprocal plot, (Y{sub max}/C{sub 0}){sup {minus}1}, may be used to quantify the {open_quotes}quality{close_quotes} of the enzyme preparation. ideally, an enzyme should have a high Y{sub max} and a low value for KC{sub 0}/Y{sub max}. The application of these assay methods to the hydrolysis of both clean, microcrystallite cellulose (Sigmacell 50) and pretreated aspen wood metal will be presented.
- OSTI ID:
- 141960
- Report Number(s):
- CONF-930304-; TRN: 93:003688-1649
- Resource Relation:
- Conference: 205. American Chemical Society national meeting, Denver, CO (United States), 28 Mar - 2 Apr 1993; Other Information: PBD: 1993; Related Information: Is Part Of 205th ACS national meeting; PB: 1951 p.
- Country of Publication:
- United States
- Language:
- English
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