In vitro effect of mercuric chloride and sodium selenite on chemiluminescent response of pronephros cells isolated from Tilapia, oreochromis aureus
- National Univ. of Singapore, Kent Ridge (Singapore)
Phagocytosis is a basic immunological function of mononuclear phagocytes and polymorphonuclear leukocytes. This process is a major defence mechanism in fish which involves recognition and killing of pathogenic microorganisms. It has been reported that phagocytic cells consume more oxygen and release several reactive oxygen species (ROS) during phagocytosis. This {open_quote}respiratory burst{close_quote} was first quantified by measuring the chemiluminescence (CL) emitted from human polymorphonuclear leukocytes and later in fish phagocytes. The oxygen intermediates responsible for this CL reaction include O{sub 2}{sup {minus}}, {center_dot}OH and H{sub 2}O{sub 2} which are also the major bactericidal agents in phagocytes{prime} oxygen-dependent killing process. Therefore, CL response can be used as an indicator of phagocytosis. This study is designed to examine the individual effects of mercury and selenium and also their possible interaction on CL response of fish pronephros phagocytes, because a defect in phagocytosis may predispose fish to diseases. 25 refs., 3 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 135825
- Journal Information:
- Bulletin of Environmental Contamination and Toxicology, Vol. 55, Issue 6; Other Information: PBD: Dec 1995
- Country of Publication:
- United States
- Language:
- English
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