Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis
Early and rapid detection of bovine tuberculosis (bTB) is critical to controlling the spread of this disease in cattle and other animals. Here in this study, we demonstrate the development of an immunoassay for the direct detection of the bovine bTB biomarker, lipomannan (LM) in serum using a waveguide-based optical biosensor. We apply an ultra-sensitive detection strategy developed by our team, termed lipoprotein capture, that exploits the pull-down of high-density lipoprotein (HDL) nanodiscs from cattle blood that allows for the recovery and detection of associated LM. We also profile the change in the expression of these TB biomarkers as a function of time from a small set of samples collected from studies of bovine TB-infected cattle. Lastly, we demonstrate for the first time the direct detection of bovine LM in serum, and clearly show that the biomarker is expressed in detectable concentrations during the entire course of the infection.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Organization:
- USDOE Laboratory Directed Research and Development (LDRD) Program; New Mexico Small Business Assistance (NMSBA); US Dept. of Agriculture (USDA)
- Grant/Contract Number:
- AC52-06NA25396
- OSTI ID:
- 1352430
- Report Number(s):
- LA-UR-17-21083
- Journal Information:
- Analytical Sciences, Vol. 33, Issue 4; ISSN 0910-6340
- Publisher:
- Japan Society for Analytical ChemistryCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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