Evaluation of hybridization efficiencies of centromeric probes for chromosomes X, Y, 18 and 13/21 on uncultured amniocytes by a `rapid one-step` fluorescence in situ hybridization
- Aprogenex, Inc., Houston, TX (United States); and others
Aprogenex has developed a rapid one-step FISH assay, KaryoSite{trademark}, for the detection of chromosomes X, Y, 13, 18 and 21. The chromosome probes used are synthetic oligonucleotides with covalently attached chromosomes. In combination with a unique hybridization cocktail, these chromosomes are detected in a one-step hybridization. This method allows the detection of aneuploidy for these five chromosomes in uncultured amniocytes as well as metaphase chromosome preparations. Hybridization efficiency of dual probe combinations, X-Rhodamine plus Y-FITC and 18-FITC plus 13/21-Rhodamine, was determined on 50 amniocyte samples. Probes were added to the cells on slides and target cellular DNA was denatured at 90{degrees}C for 5 minutes followed by hybridization at 42{degrees}C for 30 minutes. Hybridization efficiency for SY probes was greater than 95% and for 18-13/21 probes was greater than 90%. Aneuploidy data from amniocyte samples will be presented. Aprogenex`s FISH technology is a reliable and rapid one-step method for prenatal diagnosis of aneuploidy for chromosome X, Y, 13, 18 and 21 in uncultured amniocytes.
- OSTI ID:
- 134829
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-1568
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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