Dissecting the functional significance of non-catalytic carbohydrate binding modules in the deconstruction of plant cell walls
- Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
The project seeks to investigate the mechanism by which CBMs potentiate the activity of glycoside hydrolases against complete plant cell walls. The project is based on the hypothesis that the wide range of CBMs present in bacterial enzymes maximize the potential target substrates by directing the cognate enzymes not only to different regions of a specific plant cell wall, but also increases the range of plant cell walls that can be degraded. In addition to maximizing substrate access, it was also proposed that CBMs can target specific subsets of hydrolases with complementary activities to the same region of the plant cell wall, thereby maximizing the synergistic interactions between these enzymes. This synergy is based on the premise that the hydrolysis of a specific polysaccharide will increase the access of closely associated polymers to enzyme attack. In addition, it is unclear whether the catalytic module and appended CBM of modular enzymes have evolved unique complementary activities.
- Research Organization:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- DOE Contract Number:
- SC0001631
- OSTI ID:
- 1346935
- Report Number(s):
- DOE-UGA-01631; FG02-10ER16076
- Country of Publication:
- United States
- Language:
- English
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