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Title: A fine structure genomic map of the region of 12q13 containing SAS and CDK4

Abstract

We have recently adapted a method, originally described by Rackwitz, to the rapid restriction mapping of multiple cosmid DNA samples. Linearization of the cosmids at the lambda cohesive site using lambda terminase is followed by partial digestion with selected restriction enzymes and hybridization to oligonucleotides specific for the right or left hand termini. Partial digestions are performed in a microtiter plate thus allowing up to 12 cosmid clones to be digested with one restriction enzyme. We have applied this rapid restriction mapping method to cosmids derived from a region of chromosome 12q13 that has recently been shown to be amplified in a variety of cancers including malignant fibrous histiocytoma, fibrosarcoma, liposarcoma, osteosarcoma and brain tumors. A small segment of this amplification unit containing three genes, SAS (a membrane protein), CDK4 (a cyclin dependent kinase) and OS-9 (a recently described cDNA) has been analyzed with the system described above. This fine structure genomic map will be useful for completing the expression map of this region as well as characterizing its pattern of amplification in tumor specimens.

Authors:
; ;  [1]
  1. National Center for Human Genome Research, Bethesda, MD (United States); and others
Publication Date:
OSTI Identifier:
134437
Report Number(s):
CONF-941009-
Journal ID: AJHGAG; ISSN 0002-9297; TRN: 95:005313-1171
Resource Type:
Journal Article
Journal Name:
American Journal of Human Genetics
Additional Journal Information:
Journal Volume: 55; Journal Issue: Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; GENETIC MAPPING; DNA-CLONING; GENE AMPLIFICATION; HUMAN CHROMOSOME 12; STRUCTURE-ACTIVITY RELATIONSHIPS; NEOPLASMS; PATIENTS; PROTEINS; PHOSPHOTRANSFERASES; ENZYMES; OLIGONUCLEOTIDES; DNA HYBRIDIZATION

Citation Formats

Linder, C Y, Elkahloun, A G, and Su, Y A. A fine structure genomic map of the region of 12q13 containing SAS and CDK4. United States: N. p., 1994. Web.
Linder, C Y, Elkahloun, A G, & Su, Y A. A fine structure genomic map of the region of 12q13 containing SAS and CDK4. United States.
Linder, C Y, Elkahloun, A G, and Su, Y A. 1994. "A fine structure genomic map of the region of 12q13 containing SAS and CDK4". United States.
@article{osti_134437,
title = {A fine structure genomic map of the region of 12q13 containing SAS and CDK4},
author = {Linder, C Y and Elkahloun, A G and Su, Y A},
abstractNote = {We have recently adapted a method, originally described by Rackwitz, to the rapid restriction mapping of multiple cosmid DNA samples. Linearization of the cosmids at the lambda cohesive site using lambda terminase is followed by partial digestion with selected restriction enzymes and hybridization to oligonucleotides specific for the right or left hand termini. Partial digestions are performed in a microtiter plate thus allowing up to 12 cosmid clones to be digested with one restriction enzyme. We have applied this rapid restriction mapping method to cosmids derived from a region of chromosome 12q13 that has recently been shown to be amplified in a variety of cancers including malignant fibrous histiocytoma, fibrosarcoma, liposarcoma, osteosarcoma and brain tumors. A small segment of this amplification unit containing three genes, SAS (a membrane protein), CDK4 (a cyclin dependent kinase) and OS-9 (a recently described cDNA) has been analyzed with the system described above. This fine structure genomic map will be useful for completing the expression map of this region as well as characterizing its pattern of amplification in tumor specimens.},
doi = {},
url = {https://www.osti.gov/biblio/134437}, journal = {American Journal of Human Genetics},
number = Suppl.3,
volume = 55,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 1994},
month = {Thu Sep 01 00:00:00 EDT 1994}
}