Physical mapping of the congenital chloride diarrhea gene region in human chromosome 7
Abstract
The gene for congenital chloride diarrhea (CLD; MIM 214700) has been mapped to human chromosome 7 by a linkage study in Finnish families. The markers closest to the gene are D7S496 and D7S501, both with zero recombination fraction. In order to physically map the region and facilitate positional cloning, altogether 25 YAC clones have been isolated from the Washington University chromosome 7 collection of YACs. The clones form 2 contigs, 700 to 900 kb in size, around D7S496 and D7SS01. One YAC from the CEPH collection that bridges these contigs has been identified, but the link remains unconfirmed. Rare-cutter restriction mapping has identified as least 3 CpG islands within 50 to 200 kb of D7S496, supposed to map closest to CLD on the basis of linkage disequilibrium studies. Isolation of candidate cDNAs is in progress.
- Authors:
-
- Univ. of Helsinki (Finland); and others
- Publication Date:
- OSTI Identifier:
- 134424
- Report Number(s):
- CONF-941009-
Journal ID: AJHGAG; ISSN 0002-9297; TRN: 95:005313-1158
- Resource Type:
- Journal Article
- Journal Name:
- American Journal of Human Genetics
- Additional Journal Information:
- Journal Volume: 55; Journal Issue: Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; GENETIC MAPPING; DNA-CLONING; HUMAN CHROMOSOME 7; CONGENITAL DISEASES; PATIENTS; HEREDITARY DISEASES; FINLAND; STATISTICS; BIOLOGICAL MARKERS; NUCLEOTIDES; YEASTS; CONTIGS
Citation Formats
Kere, J, Hoeglund, P, and Haila, S. Physical mapping of the congenital chloride diarrhea gene region in human chromosome 7. United States: N. p., 1994.
Web.
Kere, J, Hoeglund, P, & Haila, S. Physical mapping of the congenital chloride diarrhea gene region in human chromosome 7. United States.
Kere, J, Hoeglund, P, and Haila, S. 1994.
"Physical mapping of the congenital chloride diarrhea gene region in human chromosome 7". United States.
@article{osti_134424,
title = {Physical mapping of the congenital chloride diarrhea gene region in human chromosome 7},
author = {Kere, J and Hoeglund, P and Haila, S},
abstractNote = {The gene for congenital chloride diarrhea (CLD; MIM 214700) has been mapped to human chromosome 7 by a linkage study in Finnish families. The markers closest to the gene are D7S496 and D7S501, both with zero recombination fraction. In order to physically map the region and facilitate positional cloning, altogether 25 YAC clones have been isolated from the Washington University chromosome 7 collection of YACs. The clones form 2 contigs, 700 to 900 kb in size, around D7S496 and D7SS01. One YAC from the CEPH collection that bridges these contigs has been identified, but the link remains unconfirmed. Rare-cutter restriction mapping has identified as least 3 CpG islands within 50 to 200 kb of D7S496, supposed to map closest to CLD on the basis of linkage disequilibrium studies. Isolation of candidate cDNAs is in progress.},
doi = {},
url = {https://www.osti.gov/biblio/134424},
journal = {American Journal of Human Genetics},
number = Suppl.3,
volume = 55,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 1994},
month = {Thu Sep 01 00:00:00 EDT 1994}
}