Cytogenetically uninformative or Ph{sup 1} negative patients with CML should be evaluated by FISH with bcr/abl probes
- Univ. of Utah School of Medicine, Salt Lake City, UT (United States); and others
Identification of the Ph{sup 1} translocation in chronic myelogenous leukemia (CML) is both diagnostically and prognostically important. Our laboratory has established a policy for the use of fluorescence in situ hybridization (FISH) with the Mbcr/abl probe (Oncor) to ensure that the most complete information available is obtained. Bone marrow or unstimulated peripheral blood is cultured, harvested and analyzed according to routine laboratory protocol. If the patient is Ph{sup 1} positive, the case is completed and results given with no further study. If the patient is Ph{sup 1} negative or there are no discernible metaphase cells, the physician is contacted for authorization of FISH with the Mbcr/abl probe. Clinicians are told that FISH is an investigational procedure, if the patient fits a CML profile, this test is offered. In a group of 28 CML patients for which FISH was done, 9 (32%) cases were directly affected by FISH results. Four cases contained no metaphase cells, and interphase FISH showed two of them to be Ph{sup 1} positive and two to be Ph{sup 1} negative. Three other cases were cytogenetically Ph{sup 1} negative and were Ph{sup 1} positive by FISH. Another case with one metaphase cell which was cytogenetically Ph{sup 1} negative was also negative by FISH, and a case done post-bone marrow transplant was Ph{sup 1} negative by both methods. These data show that completing a FISH study with the Mbcr/abl probe in cytogenetically Ph{sup 1} negative CML patients can significantly affect interpretation. The results of this series indicate that FISH is an important component of a complete work-up for the patient with CML.
- OSTI ID:
- 133479
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-0207
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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