Loss of chromosome 17 regions in early-stage prostate tumors detected by quantitative PCR and single-copy fluorescence in situ hybridization: Involvement of the BRCA-1 locus?
- Univ. of Utah School of Medicine, Salt Lake City, UT (United States); and others
Prostate cancer is the leading male malignancy in the U.S., yet no consistent cellular defect has been associated with the disease. Loss of whole chromosome 17 has been observed in primary prostate tumors by fluorescence in situ hybridization (FISH). A small subset of late-stage tumors showed abnormalities in the p53 gene on 17p, but no loss of heterozygosity (LOH) has been observed on the long arm of 17 in prostate cancer. An increased incidence of prostate cancers in families with linkage to the familial breast-ovarian cancer predisposition gene, BRCA-1, indicates that this locus, which maps to 17q, may also be involved in prostate cancers. We studied 23 early-stage primary prostate tumors, obtained from radical prostatectomies, for LOH at five initial sites on chromosome 17 using highly informative microsatellite markers and quantitative analysis of polymerase chain reaction (PCR) product. Six of the 23 specimens (26%) showed tumor-specific LOH on chromosome 17. Two specimens showed interstitial loss near the BRCA-1 region on 17q. We confirmed these losses by single copy FISH on fixed cell suspensions from the primary tumors using selected P1 probes flanking the markers. Our findings suggest that genes on proximal 17q may play a pivotal role in the development of at least a subset of prostate tumors.
- OSTI ID:
- 133478
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; CNN: Grant R01-CA46269; Grant M01-RR00064; TRN: 95:005313-0206
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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