Effect of fed-batch vs. continuous mode of operation on microbial fuel cell performance treating biorefinery wastewater

Pannell, Tyler C.; Goud, R. Kannaiah; Schell, Daniel J.; Borole, Abhijeet P.

doi:10.1016/j.bej.2016.04.029

Title: Effect of fed-batch vs. continuous mode of operation on microbial fuel cell performance treating biorefinery wastewater

Journal Article · Mon May 02 00:00:00 EDT 2016 · Biochemical Engineering Journal

DOI:https://doi.org/10.1016/j.bej.2016.04.029· OSTI ID:1332910

Pannell, Tyler C. ^[1]; Goud, R. Kannaiah ^[1]; Schell, Daniel J. ^[2]; Borole, Abhijeet P. ^[3]

The Univ. of Tennessee, Knoxville, TN (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
The Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Bioelectrochemical systems have been shown to treat low-value biorefinery streams while recovering energy, however, low current densities and anode conversion efficiencies (ACE) limit their application. A bioanode was developed via enrichment of electroactive biofilm under fed-batch and continuous feeding conditions using corn stover-derived waste stream. The continuously-fed MFC exhibited a current density of 5.8 ± 0.06 A/m² and an ACE of 39% ± 4. The fed-batch MFC achieved a similar current density and an ACE of 19.2%, however, its performance dropped after 36 days of operation to 1.1 A/m² and 0.5%, respectively. In comparison, the ACE of the continuously-fed MFC remained stable achieving an ACE of 30% ± 3 after 48 days of operation. An MFC treating a biorefinery stream post fuel separation achieved a current density of 10.7 ± 0.1 A/m² and an ACE of 57% ± 9 at an organic loading of 12.5 g COD/L-day. Characterization of the microbial communities indicate higher abundance of Firmicutes and Proteobacteria and lower abundance of Bacteriodetes and a higher level of Geobacter spp. (1.4% vs. 0.2%) in continuously-fed MFC vs. fed-batch MFC. Finally, the results demonstrate that limiting substrate to the equivalent maximum current that the anode can generate, maintains MFC performance over a long term for high strength wastewaters, such as those generated in the biorefinery.

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Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office; Work for Others (WFO); USDOE Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: AC36-08GO28308; AC05-00OR22725

OSTI ID:: 1332910

Alternate ID(s):: OSTI ID: 1263833; OSTI ID: 1410819

Report Number(s):: NREL/JA-5100-66631

Journal Information:: Biochemical Engineering Journal, Vol. 116; ISSN 1369-703X

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 38 works

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References (33)

Effect of external resistance on bacterial diversity and metabolism in cellulose-fed microbial fuel cells Rismani-Yazdi, Hamid; Christy, Ann D.; Carver, Sarah M. Bioresource Technology, Vol. 102, Issue 1 https://doi.org/10.1016/j.biortech.2010.05.012	journal	January 2011
Comparison of anode bacterial communities and performance in microbial fuel cells with different electron donors Jung, Sokhee; Regan, John M. Applied Microbiology and Biotechnology, Vol. 77, Issue 2 https://doi.org/10.1007/s00253-007-1162-y	journal	September 2007
Hydrogen production from switchgrass via an integrated pyrolysis–microbial electrolysis process Lewis, A. J.; Ren, S.; Ye, X. Bioresource Technology, Vol. 195 https://doi.org/10.1016/j.biortech.2015.06.085	journal	November 2015
Electroactive biofilms: Current status and future research needs Borole, Abhijeet P.; Reguera, Gemma; Ringeisen, Bradley Energy & Environmental Science, Vol. 4, Issue 12 https://doi.org/10.1039/c1ee02511b	journal	January 2011
Phylogeny of Microorganisms Populating a Thick, Subaerial, Predominantly Lithotrophic Biofilm at an Extreme Acid Mine Drainage Site Bond, P. L.; Smriga, S. P.; Banfield, J. F. Applied and Environmental Microbiology, Vol. 66, Issue 9 https://doi.org/10.1128/AEM.66.9.3842-3849.2000	journal	September 2000
Improving energy efficiency and enabling water recycling in biorefineries using bioelectrochemical systems† Borole, Abhijeet P. Biofuels, Bioproducts and Biorefining, Vol. 5, Issue 1 https://doi.org/10.1002/bbb.265	journal	January 2011
Phenol oxidase activity in bacteria of the genus Azospirillum Nikitina, V. E.; Vetchinkina, E. P.; Ponomareva, E. G. Microbiology, Vol. 79, Issue 3 https://doi.org/10.1134/S0026261710030082	journal	June 2010
Microbial fuel cells: novel biotechnology for energy generation Rabaey, Korneel; Verstraete, Willy Trends in Biotechnology, Vol. 23, Issue 6 https://doi.org/10.1016/j.tibtech.2005.04.008	journal	June 2005
Continuous power generation and microbial community structure of the anode biofilms in a three-stage microbial fuel cell system Chung, Kyungmi; Okabe, Satoshi Applied Microbiology and Biotechnology, Vol. 83, Issue 5 https://doi.org/10.1007/s00253-009-1990-z	journal	July 2009
Electricity generation from model organic wastewater in a cassette-electrode microbial fuel cell Shimoyama, Takefumi; Komukai, Shoko; Yamazawa, Akira Applied Microbiology and Biotechnology, Vol. 80, Issue 2 https://doi.org/10.1007/s00253-008-1516-0	journal	August 2008
Effect of different substrates on the performance, bacterial diversity, and bacterial viability in microbial fuel cells Chae, Kyu-Jung; Choi, Mi-Jin; Lee, Jin-Wook Bioresource Technology, Vol. 100, Issue 14 https://doi.org/10.1016/j.biortech.2009.02.065	journal	July 2009
Improved performance of porous bio-anodes in microbial electrolysis cells by enhancing mass and charge transport Sleutels, Tom H. J. A.; Lodder, Rob; Hamelers, Hubertus V. M. International Journal of Hydrogen Energy, Vol. 34, Issue 24 https://doi.org/10.1016/j.ijhydene.2009.09.089	journal	December 2009
Controlling accumulation of fermentation inhibitors in biorefinery recycle water using microbial fuel cells Borole, Abhijeet P.; Mielenz, Jonathan R.; Vishnivetskaya, Tatiana A. Biotechnology for Biofuels, Vol. 2, Issue 1 https://doi.org/10.1186/1754-6834-2-7	journal	January 2009
Effects of Substrate Diffusion and Anode Potential on Kinetic Parameters for Anode-Respiring Bacteria Lee, Hyung-Sool; Torres, César I.; Rittmann, Bruce E. Environmental Science & Technology, Vol. 43, Issue 19 https://doi.org/10.1021/es9015519	journal	October 2009
Enhancement in current density and energy conversion efficiency of 3-dimensional MFC anodes using pre-enriched consortium and continuous supply of electron donors Borole, Abhijeet P.; Hamilton, Choo Y.; Vishnivetskaya, Tatiana A. Bioresource Technology, Vol. 102, Issue 8 https://doi.org/10.1016/j.biortech.2011.01.045	journal	April 2011
Electricity generation from cellulose by rumen microorganisms in microbial fuel cells Rismani-Yazdi, Hamid; Christy, Ann D.; Dehority, Burk A. Biotechnology and Bioengineering, Vol. 97, Issue 6 https://doi.org/10.1002/bit.21366	journal	January 2007
Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysate Mohagheghi, Ali; Dowe, Nancy; Schell, Daniel Biotechnology Letters, Vol. 26, Issue 4 https://doi.org/10.1023/B:BILE.0000015451.96737.96	journal	February 2004
Change in microbial communities in acetate- and glucose-fed microbial fuel cells in the presence of light Xing, Defeng; Cheng, Shaoan; Regan, John M. Biosensors and Bioelectronics, Vol. 25, Issue 1 https://doi.org/10.1016/j.bios.2009.06.013	journal	September 2009
Estimating hydrogen production potential in biorefineries using microbial electrolysis cell technology Borole, Abhijeet P.; Mielenz, Jonathan R. International Journal of Hydrogen Energy, Vol. 36, Issue 22 https://doi.org/10.1016/j.ijhydene.2011.03.152	journal	November 2011
Metagenome and metaproteome analyses of microbial communities in mesophilic biogas-producing anaerobic batch fermentations indicate concerted plant carbohydrate degradation Hanreich, Angelika; Schimpf, Ulrike; Zakrzewski, Martha Systematic and Applied Microbiology, Vol. 36, Issue 5 https://doi.org/10.1016/j.syapm.2013.03.006	journal	July 2013
High-Performance Bioanode Development for Fermentable Substrates via Controlled Electroactive Biofilm Growth Ichihashi, Osamu; Vishnivetskaya, Tatiana A.; Borole, Abhijeet P. ChemElectroChem, Vol. 1, Issue 11 https://doi.org/10.1002/celc.201402206	journal	October 2014
Integrating engineering design improvements with exoelectrogen enrichment process to increase power output from microbial fuel cells Borole, Abhijeet P.; Hamilton, Choo Y.; Vishnivetskaya, Tatiana A. Journal of Power Sources, Vol. 191, Issue 2 https://doi.org/10.1016/j.jpowsour.2009.02.006	journal	June 2009
Development of a high-throughput method to evaluate the impact of inhibitory compounds from lignocellulosic hydrolysates on the growth of Zymomonas mobilis Franden, Mary Ann; Pienkos, Philip T.; Zhang, Min Journal of Biotechnology, Vol. 144, Issue 4 https://doi.org/10.1016/j.jbiotec.2009.08.006	journal	December 2009
Microbial community structure in a biofilm anode fed with a fermentable substrate: The significance of hydrogen scavengers Parameswaran, Prathap; Zhang, Husen; Torres, CÃ©sar I. Biotechnology and Bioengineering, Vol. 105, Issue 1 https://doi.org/10.1002/bit.22508	journal	January 2010
Low Substrate Loading Limits Methanogenesis and Leads to High Coulombic Efficiency in Bioelectrochemical Systems Sleutels, Tom; Molenaar, Sam; Heijne, Annemiek Microorganisms, Vol. 4, Issue 1 https://doi.org/10.3390/microorganisms4010007	journal	January 2016
Functionally Stable and Phylogenetically Diverse Microbial Enrichments from Microbial Fuel Cells during Wastewater Treatment Ishii, Shun'ichi; Suzuki, Shino; Norden-Krichmar, Trina M. PLoS ONE, Vol. 7, Issue 2 https://doi.org/10.1371/journal.pone.0030495	journal	February 2012
Biocatalysts in microbial fuel cells Sharma, Vinay; Kundu, P. P. Enzyme and Microbial Technology, Vol. 47, Issue 5 https://doi.org/10.1016/j.enzmictec.2010.07.001	journal	October 2010
Conversion of Residual Organics in Corn Stover-Derived Biorefinery Stream to Bioenergy via a Microbial Fuel Cell Borole, Abhijeet P.; Hamilton, Choo Y.; Schell, Daniel J. Environmental Science & Technology, Vol. 47, Issue 1 https://doi.org/10.1021/es3023495	journal	August 2012
Effect of operational parameters on Coulombic efficiency in bioelectrochemical systems Sleutels, Tom H. J. A.; Darus, Libertus; Hamelers, Hubertus V. M. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.09.078	journal	December 2011
High shear enrichment improves the performance of the anodophilic microbial consortium in a microbial fuel cell Pham, Hai The; Boon, Nico; Aelterman, Peter Microbial Biotechnology, Vol. 1, Issue 6 https://doi.org/10.1111/j.1751-7915.2008.00049.x	journal	November 2008
Improving power production in acetate-fed microbial fuel cells via enrichment of exoelectrogenic organisms in flow-through systems Borole, Abhijeet P.; Hamilton, Choo Y.; Vishnivetskaya, Tatiana Biochemical Engineering Journal, Vol. 48, Issue 1 https://doi.org/10.1016/j.bej.2009.08.008	journal	December 2009
Evaluation of energy-conversion efficiencies in microbial fuel cells (MFCs) utilizing fermentable and non-fermentable substrates Lee, Hyung-Sool; Parameswaran, Prathap; Kato-Marcus, Andrew Water Research, Vol. 42, Issue 6-7 https://doi.org/10.1016/j.watres.2007.10.036	journal	March 2008
Evaluation of hydrolysis and fermentation rates in microbial fuel cells Velasquez-Orta, Sharon B.; Yu, Eileen; Katuri, Krishna P. Applied Microbiology and Biotechnology, Vol. 90, Issue 2 https://doi.org/10.1007/s00253-011-3126-5	journal	February 2011

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Storing of exoelectrogenic anolyte for efficient microbial fuel cell recovery Haavisto, Johanna M.; Lakaniemi, Aino-Maija; Puhakka, Jaakko A. Taylor & Francis https://doi.org/10.6084/m9.figshare.5793852	text	January 2018
Concentration Pulse Method for the Investigation of Transformation Pathways in a Glycerol-Fed Bioelectrochemical System Kubannek, Fabian; Moß, Christopher; Huber, Katharina Frontiers in Energy Research, Vol. 6 https://doi.org/10.3389/fenrg.2018.00125	journal	November 2018
Storing of exoelectrogenic anolyte for efficient microbial fuel cell recovery Haavisto, Johanna M.; Lakaniemi, Aino-Maija; Puhakka, Jaakko A. Taylor & Francis https://doi.org/10.6084/m9.figshare.5793852.v1	text	January 2018
Storing of exoelectrogenic anolyte for efficient microbial fuel cell recovery Haavisto, Johanna M.; Lakaniemi, Aino-Maija; Puhakka, Jaakko A. Environmental Technology, Vol. 40, Issue 11 https://doi.org/10.1080/09593330.2017.1423395	journal	January 2018

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Related Subjects

09 BIOMASS FUELS
fermentation inhibitors
bioelectrochemical system
exoelectrogenic
biofilm-forming
hydrolysate
corn stover
59 BASIC BIOLOGICAL SCIENCES
bioelectrochemical systems
microbial fuel cells
biorefinery wastewater