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Title: A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers

Thioacidolysis is a method used to measure the relative content of lignin monomers bound by SS-O-4 linkages. Current thioacidolysis methods are low-throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non-chlorinated organic solvent and is tailored for higher-throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1-2 mg of biomass per assay and has been quantified using fast-GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day-to-day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. The method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.
 [1] ;  [2] ;  [1] ;  [1] ;  [2] ;  [2] ;  [3] ;  [3] ;  [1]
  1. Bioenergy Science Center, Golden CO USA; National Bioenergy Center, National Renewable Energy Laboratory, Golden CO USA
  2. Great Lakes BioEnergy Research Center, Michigan State University, East Lansing MI USA
  3. Wisconsin Bioenergy Initiative, University of Wisconsin, Madison WI USA
Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 1860-6768
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biotechnology Journal; Journal Volume: 11; Journal Issue: 10
Research Org:
NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))
Sponsoring Org:
USDOE Office of Science (SC), Office of Biological and Environmental Research
Country of Publication:
United States
09 BIOMASS FUELS cell wall structure; lignin; S/G ratio; thioacidolysis