High Resolution Mapping of Bactericidal Monoclonal Antibody Binding Epitopes on Staphylococcus aureus Antigen MntC

Gribenko, Alexey V.; Parris, Kevin; Mosyak, Lidia; Li, Sheng; Handke, Luke; Hawkins, Julio C.; Severina, Elena; Matsuka, Yury V.; Anderson, Annaliesa S.; Zhang, Gongyi

doi:10.1371/journal.ppat.1005908

Title: High Resolution Mapping of Bactericidal Monoclonal Antibody Binding Epitopes on Staphylococcus aureus Antigen MntC

Journal Article · Fri Sep 30 00:00:00 EDT 2016 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1005908· OSTI ID:1328023

Gribenko, Alexey V. ^[1]; Parris, Kevin ^[2]; Mosyak, Lidia ^[2]; Li, Sheng ^[3]; Handke, Luke ^[1]; Hawkins, Julio C. ^[1]; Severina, Elena ^[1]; Matsuka, Yury V. ^[1]; Anderson, Annaliesa S. ^[1]; Zhang, Gongyi ^[4]

Pfizer Vaccine Research and Development, Pearl River, NY (United States)
Pfizer Protein Engineering and Production, Cambridge, MA (United States)
Univ. of California at San Diego, La Jolla, CA (United States)
National Jewish Health, Denver, CO (United States)

The Staphylococcus aureus manganese transporter protein MntC is under investigation as a component of a prophylactic S.aureus vaccine. Passive immunization with monoclonal antibodies mAB 305-78-7 and mAB 305-101-8 produced using MntC was shown to significantly reduce S. aureus burden in an infant rat model of infection. Earlier interference mapping suggested that a total of 23 monoclonal antibodies generated against MntC could be subdivided into three interference groups, representing three independent immunogenic regions. In the current work binding epitopes for selected representatives of each of these interference groups (mAB 305-72-5 – group 1, mAB 305-78-7 – group 2, and mAB 305-101-8 – group 3) were mapped using Hydrogen-Deuterium Exchange Mass Spectrometry (DXMS). All of the identified epitopes are discontinuous, with binding surface formed by structural elements that are separated within the primary sequence of the protein but adjacent in the context of the three-dimensional structure. The approach was validated by co-crystallizing the Fab fragment of one of the antibodies (mAB 305-78-7) with MntC and solving the three-dimensional structure of the complex. X-ray results themselves and localization of the mAB 305-78-7 epitope were further validated using antibody binding experiments with MntC variants containing substitutions of key amino acid residues. These results provided insight into the antigenic properties of MntC and how these properties may play a role in protecting the hostagainst S. aureus infection by preventing the capture and transport of Mn²⁺, a key element that the pathogen uses to evade host immunity.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE

OSTI ID:: 1328023

Journal Information:: PLoS Pathogens, Vol. 12, Issue 9; ISSN 1553-7374

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 21 works

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59 BASIC BIOLOGICAL SCIENCES
deuterium
monoclonal antibodies
antibodies
epitope mapping
X-ray crystallography
enzyme-linked immunoassays
amino acid substitution
Staphylococcus aureus

Title: High Resolution Mapping of Bactericidal Monoclonal Antibody Binding Epitopes on Staphylococcus aureus Antigen MntC

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