Natural genetic variability reduces recalcitrance in poplar

Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev; Tuskan, Gerald A.; Wyman, Charles E.

doi:10.1186/s13068-016-0521-2

Title: Natural genetic variability reduces recalcitrance in poplar

Journal Article · Fri May 20 00:00:00 EDT 2016 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-016-0521-2· OSTI ID:1618640

Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev; Tuskan, Gerald A.; Wyman, Charles E.

Here, lignin content and structure are known to affect recalcitrance of lignocellulosic biomass to chemical/biochemical conversion. Previously, we identified rare Populus trichocarpa natural variants with significantly reduced lignin content. Because reduced lignin content may lower recalcitrance, 18 rare variants along with 4 comparators, and BESC standard Populus was analyzed for composition of structural carbohydrates and lignin. Sugar yields from these plants were measured at 5 process conditions: one for just enzymatic hydrolysis without pretreatment and four via our combined high-throughput hot water pretreatment and co-hydrolysis (HTPH) technique.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1618640

Alternate ID(s):: OSTI ID: 1327698

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 9 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

Citation Metrics:

Cited by: 29 works

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Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems Thomas, Vanessa A.; Kothari, Ninad; Bhagia, Samarthya Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0975-x	journal	November 2017
Bridging the gap between feedstock growers and users: the study of a coppice poplar-based biorefinery Dou, Chang; Gustafson, Rick; Bura, Renata Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1079-y	journal	March 2018
Sugar yield and composition of tubers from Jerusalem Artichoke ( Helianthus tuberosus ) irrigated with saline waters Bhagia, Samarthya; Ferreira, Jorge F. S.; Kothari, Ninad Biotechnology and Bioengineering, Vol. 115, Issue 6 https://doi.org/10.1002/bit.26582	journal	March 2018
Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis Wang, Jack P.; Matthews, Megan L.; Williams, Cranos M. Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-03863-z	journal	April 2018
Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii Straub, Christopher T.; Khatibi, Piyum A.; Wang, Jack P. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-11376-6	journal	August 2019
Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii Zurawski, Jeffrey V.; Khatibi, Piyum A.; Akinosho, Hannah O. Applied and Environmental Microbiology, Vol. 83, Issue 17 https://doi.org/10.1128/aem.00969-17	journal	June 2017
Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction Conway, Jonathan M.; McKinley, Bennett S.; Seals, Nathaniel L. Applied and Environmental Microbiology, Vol. 83, Issue 24 https://doi.org/10.1128/aem.01828-17	journal	October 2017
Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood Johnson, Amanda M.; Kim, Hoon; Ralph, John Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0734-z	journal	February 2017
Multiple levers for overcoming the recalcitrance of lignocellulosic biomass Holwerda, Evert K.; Worthen, Robert S.; Kothari, Ninad Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1353-7	journal	January 2019
Emerging Technologies for the Production of Renewable Liquid Transport Fuels from Biomass Sources Enriched in Plant Cell Walls Tan, Hwei-Ting; Corbin, Kendall R.; Fincher, Geoffrey B. Frontiers in Plant Science, Vol. 7 https://doi.org/10.3389/fpls.2016.01854	journal	December 2016
The Economics of Rapid Multiplication of Hybrid Poplar Biomass Varieties Stanton, Brian J.; Haiby, Kathy; Gantz, Carlos Forests, Vol. 10, Issue 5 https://doi.org/10.3390/f10050446	journal	May 2019

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Natural genetic variability reduces recalcitrance in poplar Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev https://doi.org/10.6084/m9.figshare.c.3603218 The current record is supplemented by this dataset	dataset	May 2016
MOESM1 of Natural genetic variability reduces recalcitrance in poplar Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev https://doi.org/10.6084/m9.figshare.c.3603218_d1.v1 The current record is supplemented by this dataset	dataset	May 2016
Natural genetic variability reduces recalcitrance in poplar Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev https://doi.org/10.6084/m9.figshare.c.3603218.v1 The current record is supplemented by this dataset	dataset	May 2016
MOESM1 of Natural genetic variability reduces recalcitrance in poplar Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev https://doi.org/10.6084/m9.figshare.c.3603218_d1 The current record is supplemented by this text	text	January 2016