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Title: Conventional and Advanced Separations in Mass Spectrometry-Based Metabolomics: Methodologies and Applications

Metabolomics is the quantitative analysis of all metabolites in a given sample. Due to the chemical complexity of the metabolome, optimal separations are required for comprehensive identification and quantification of sample constituents. This chapter provides an overview of both conventional and advanced separations methods in practice for reducing the complexity of metabolite extracts delivered to the mass spectrometer detector, and covers gas chromatography (GC), liquid chromatography (LC), capillary electrophoresis (CE), supercritical fluid chromatography (SFC) and ion mobility spectrometry (IMS) separation techniques coupled with mass spectrometry (MS) as both uni-dimensional and as multi-dimensional approaches.
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Publication Date:
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Report Number(s):
48679; KP1601010
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Related Information: Reference Module in Chemistry, Molecular Sciences and Chemical Engineering
Reedijk, J; Elsevier, Amsterdam, ZZ, Netherlands.
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
Country of Publication:
United States
capillary electrophoresis – mass spectrometry (CE-MS); coupled separation; gas chromatography – mass spectrometry (GC-MS); GCxGC – MS; ion mobility spectrometry (IMS); LCxLC – MS; liquid chromatography – mass spectrometry (LC-MS); metabolomics; multi-dimensional chromatography; supercritical fluid chromatography – mass spectrometry (SFC – MS); Environmental Molecular Sciences Laboratory