A multi-omic future for microbiome studies
Abstract
Microbes constitute about a third of the Earth’s biomass and play critical roles in sustaining life. While results from multiple sequence-based studies have illustrated the importance of microbial communities for human health and the environment, additional technological developments are still needed to gain more insight into their functions [1]. To date, the majority of sequencing studies have focused on the 16S rRNA gene as a phylogenetic marker. This approach has enabled exploration of microbial compositions in a range of sample types, while bypassing the need for cultivation. 16S rRNA gene sequencing has also enabled a vast majority of microorganisms never previously isolated in culture to be identified and placed into a phylogenetic context [2]. These technologies have been utilized to map the locations of microbes inhabiting various locations of the body [3]. Similarly, sequencing has been used to determine the identities and distributions of microorganisms inhabiting different ecosystems [4, 5], and efforts in single cell sequencing of the microbiome have helped fill in missing branches of the phylogenetic tree [6].
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1327110
- Report Number(s):
- PNNL-SA-116189
Journal ID: ISSN 2058-5276; 48680; 453040220
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Journal Article
- Journal Name:
- Nature Microbiology
- Additional Journal Information:
- Journal Volume: 1; Journal Issue: 5; Journal ID: ISSN 2058-5276
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Environmental Molecular Sciences Laboratory
Citation Formats
Jansson, Janet K., and Baker, Erin S. A multi-omic future for microbiome studies. United States: N. p., 2016.
Web. doi:10.1038/NMICROBIOL.2016.49.
Jansson, Janet K., & Baker, Erin S. A multi-omic future for microbiome studies. United States. https://doi.org/10.1038/NMICROBIOL.2016.49
Jansson, Janet K., and Baker, Erin S. 2016.
"A multi-omic future for microbiome studies". United States. https://doi.org/10.1038/NMICROBIOL.2016.49.
@article{osti_1327110,
title = {A multi-omic future for microbiome studies},
author = {Jansson, Janet K. and Baker, Erin S.},
abstractNote = {Microbes constitute about a third of the Earth’s biomass and play critical roles in sustaining life. While results from multiple sequence-based studies have illustrated the importance of microbial communities for human health and the environment, additional technological developments are still needed to gain more insight into their functions [1]. To date, the majority of sequencing studies have focused on the 16S rRNA gene as a phylogenetic marker. This approach has enabled exploration of microbial compositions in a range of sample types, while bypassing the need for cultivation. 16S rRNA gene sequencing has also enabled a vast majority of microorganisms never previously isolated in culture to be identified and placed into a phylogenetic context [2]. These technologies have been utilized to map the locations of microbes inhabiting various locations of the body [3]. Similarly, sequencing has been used to determine the identities and distributions of microorganisms inhabiting different ecosystems [4, 5], and efforts in single cell sequencing of the microbiome have helped fill in missing branches of the phylogenetic tree [6].},
doi = {10.1038/NMICROBIOL.2016.49},
url = {https://www.osti.gov/biblio/1327110},
journal = {Nature Microbiology},
issn = {2058-5276},
number = 5,
volume = 1,
place = {United States},
year = {Tue Apr 26 00:00:00 EDT 2016},
month = {Tue Apr 26 00:00:00 EDT 2016}
}