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This content will become publicly available on June 20, 2017

Title: Determining the syringyl/guaiacyl lignin ratio in the vessel and fiber cell walls of transgenic Populus plants

Observation of the spatial lignin distribution throughout the plant cell wall provides insight into the physicochemical characteristics of lignocellulosic biomass. The distribution of syringyl (S) and guaiacyl (G) lignin in cell walls of a genetically modified Populus deltoides and its corresponding empty vector control were analyzed with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and then mapped to determine the S/G lignin ratio of the sample surface and specific regions of interest (ROIs). The surface characterizations of transgenic cross-sections within 1 cm vertical distance of each other on the stem possess similar S/G lignin ratios. Furthermore, the analysis of the ROIs determined that there was a 50% decrease in the S/G lignin ratio of the transgenic xylem fiber cell walls.
 [1] ;  [1] ;  [1] ;  [1]
  1. Georgia Inst. of Technology, Atlanta, GA (United States)
Publication Date:
OSTI Identifier:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Energy and Fuels
Additional Journal Information:
Journal Volume: 30; Journal Issue: 7; Journal ID: ISSN 0887-0624
American Chemical Society (ACS)
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States