Summers_FinalReport_ER64408
- Univ. of Georgia, Athens, GA (United States)
- Genentech, San Francisco, CA (United States)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Elemental mercury, Hg(0) is a contaminant at many DOE sites, especially at Oak Ridge National Laboratory (ORNL) where the spread of spilled Hg and its effects on microbial populations have been monitored for decades. To explore the microbial interactions with Hg, we devised a global proteomic approach capable of directly detecting Hg-adducts of proteins. This technique developed in the facultative anaerobe, Escherichia coli, allows us to identify the proteins most vulnerable to acute exposure to organomercurials phenyl- and ethyl-mercury (as surrogates for the highly neurotoxic methyl-Hg) (Polacco, et al, 2011). We have found >300 such proteins in all metabolic functional groups and cellular compartments; most are highly conserved and can serve as markers for acute Hg exposure (Zink, et al. 2016, in preparation).
- Research Organization:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- FG02-07ER64408
- OSTI ID:
- 1302637
- Report Number(s):
- DOE-UGA-10-21-RR182-348
- Country of Publication:
- United States
- Language:
- English
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