Carbon dioxide "trapped" in a β-carbonic anhydrase
Abstract
Carbonic anhydrases (CAs) are enzymes that catalyze the hydration/ dehydration of CO2/HCO3- with rates approaching diffusion-controlled limits (kcat/KM ~ 108 M–1s–1). Here, this family of enzymes has evolved disparate protein folds that all perform the same reaction at near catalytic perfection. Presented here is a structural study of a beta-CA (psCA3) expressed in Pseudomonas aeruginosa, in complex with CO2, using pressurized cryocooled crystallography. The structure has been refined to 1.6 angstrom resolution with Rcryst and Rfree values of 17.3 and 19.9%, respectively, and is compared with the α-CA, human CA isoform II (hCA II), the only other CA to have CO2, captured in its active site. Despite the lack of structural similarity between psCA3 and hCA II, the CO2, binding orientation relative to the zinc-bound solvent is identical. In addition, a second CO2, binding site was located at the dimer interface of psCA3. Interestingly, all β-CAs function as dirners or higher-order oligomeric states, and the CO2, bound at the interface may contribute to the allosteric nature of this family of enzymes or may be a convenient alternative binding site as this pocket has been previously shown to be a promiscuous site for a variety of ligands, including bicarbonate, sulfate, andmore »
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Cornell Univ., Ithaca, NY (United States)
- Univ. of Florida, Gainesville, FL (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1287017
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Biochemistry
- Additional Journal Information:
- Journal Volume: 54; Journal Issue: 43; Journal ID: ISSN 0006-2960
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; β-carbonic anhydrase; Pseudomonas aeruginosa; CO2 binding
Citation Formats
Aggarwal, Mayank, Chua, Teck Khiang, Pinard, Melissa A., Szebenyi, Doletha M., and McKenna, Robert. Carbon dioxide "trapped" in a β-carbonic anhydrase. United States: N. p., 2015.
Web. doi:10.1021/acs.biochem.5b00987.
Aggarwal, Mayank, Chua, Teck Khiang, Pinard, Melissa A., Szebenyi, Doletha M., & McKenna, Robert. Carbon dioxide "trapped" in a β-carbonic anhydrase. United States. https://doi.org/10.1021/acs.biochem.5b00987
Aggarwal, Mayank, Chua, Teck Khiang, Pinard, Melissa A., Szebenyi, Doletha M., and McKenna, Robert. 2015.
"Carbon dioxide "trapped" in a β-carbonic anhydrase". United States. https://doi.org/10.1021/acs.biochem.5b00987. https://www.osti.gov/servlets/purl/1287017.
@article{osti_1287017,
title = {Carbon dioxide "trapped" in a β-carbonic anhydrase},
author = {Aggarwal, Mayank and Chua, Teck Khiang and Pinard, Melissa A. and Szebenyi, Doletha M. and McKenna, Robert},
abstractNote = {Carbonic anhydrases (CAs) are enzymes that catalyze the hydration/ dehydration of CO2/HCO3- with rates approaching diffusion-controlled limits (kcat/KM ~ 108 M–1s–1). Here, this family of enzymes has evolved disparate protein folds that all perform the same reaction at near catalytic perfection. Presented here is a structural study of a beta-CA (psCA3) expressed in Pseudomonas aeruginosa, in complex with CO2, using pressurized cryocooled crystallography. The structure has been refined to 1.6 angstrom resolution with Rcryst and Rfree values of 17.3 and 19.9%, respectively, and is compared with the α-CA, human CA isoform II (hCA II), the only other CA to have CO2, captured in its active site. Despite the lack of structural similarity between psCA3 and hCA II, the CO2, binding orientation relative to the zinc-bound solvent is identical. In addition, a second CO2, binding site was located at the dimer interface of psCA3. Interestingly, all β-CAs function as dirners or higher-order oligomeric states, and the CO2, bound at the interface may contribute to the allosteric nature of this family of enzymes or may be a convenient alternative binding site as this pocket has been previously shown to be a promiscuous site for a variety of ligands, including bicarbonate, sulfate, and phosphate ions.},
doi = {10.1021/acs.biochem.5b00987},
url = {https://www.osti.gov/biblio/1287017},
journal = {Biochemistry},
issn = {0006-2960},
number = 43,
volume = 54,
place = {United States},
year = {Mon Oct 12 00:00:00 EDT 2015},
month = {Mon Oct 12 00:00:00 EDT 2015}
}
Web of Science
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