Use of Agrobacterium rhizogenes strain 18r12v and paromomycin selection for transformation of Brachypodium distachyon and Brachypodium sylvaticum

Collier, Ray; Bragg, Jennifer; Hernandez, Bryan T.; Vogel, John P.; Thilmony, Roger

doi:10.3389/fpls.2016.00716

Title: Use of Agrobacterium rhizogenes strain 18r12v and paromomycin selection for transformation of Brachypodium distachyon and Brachypodium sylvaticum

Journal Article · Tue May 24 00:00:00 EDT 2016 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2016.00716· OSTI ID:1282007

Collier, Ray ^[1]; Bragg, Jennifer ^[1]; Hernandez, Bryan T. ^[2]; Vogel, John P. ^[3]; Thilmony, Roger ^[1]

United States Dept. of Agriculture-Agricultural Research Service, Albany, CA, (United States). Crop Improvement and Genetics Research Unit, Western Regional Research Center
Univ. of California, Davis, Davis, CA (United States). Dept. of Plant Sciences
United States Dept. of Agriculture-Agricultural Research Service, Albany, CA, (United States). Crop Improvement and Genetics Research Unit, Western Regional Research Center; Dept. of Energy, Joint Genome Institute, Walnut Creek, CA (United States)

In this study, the genetic transformation of monocot grasses is a resource intensive process, the quality and efficiency of which is dependent in part upon the method of DNA introduction, as well as the ability to effectively separate transformed from wildtype tissue. Agrobacterium-mediated transformation of Brachypodium has relied mainly on Agrobacterium tumefaciens strain AGL1. Currently the antibiotic hygromycin B has been the selective agent of choice for robust identification of transgenic calli in Brachypodium distachyon and Brachypodium sylvaticum but few other chemicals have been shown to work as well for selection of transgenic Brachypodium cells in tissue culture. This study demonstrates that Agrobacterium rhizogenes strain 18r12v and paromomycin selection can be successfully used for the efficient generation of transgenic B. distachyon and B. sylvaticurn. Additionally we observed that the transformation rates were similar to or higher than those obtained with A. turnefaciens strain AGL1 and hygromycin selection. The A. rhizogenes strain 18r12v harboring the pARS1 binary vector and paromomycin selection is an effective means of generating transgenic Brachypodium plants. This novel approach will facilitate the transgenic complementation of T-DNA knockout mutants of B. distachyon which were created using hygromycin selection, as well as aid the implementation of more complex genome manipulation strategies which require multiple rounds of transformation.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: United States Dept. of Agriculture-Agricultural Research Service, Albany, CA, (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Joint Genome Institute (JGI)

Grant/Contract Number:: SC0001526; AC02-05CH11231

OSTI ID:: 1282007

Journal Information:: Frontiers in Plant Science, Vol. 7; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 12 works

Citation information provided by
Web of Science

References (28)

A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation Bevan, Michael W.; Flavell, Richard B.; Chilton, Mary-Dell Nature, Vol. 304, Issue 5922 https://doi.org/10.1038/304184a0	journal	July 1983
Brachypodium distachyon Bragg, Jennifer N.; Anderton, Amy; Nieu, Rita Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-1695-5_2	book	September 2014
Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection Bragg, Jennifer N.; Wu, Jiajie; Gordon, Sean P. PLoS ONE, Vol. 7, Issue 9 https://doi.org/10.1371/journal.pone.0041916	journal	September 2012
Gene transfer to plants by diverse species of bacteria Broothaerts, Wim; Mitchell, Heidi J.; Weir, Brian Nature, Vol. 433, Issue 7026 https://doi.org/10.1038/nature03309	journal	February 2005
Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants Christensen, Alan H.; Quail, Peter H. Transgenic Research, Vol. 5, Issue 3 https://doi.org/10.1007/BF01969712	journal	May 1996
Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation Christensen, Alan H.; Sharrock, Robert A.; Quail, Peter H. Plant Molecular Biology, Vol. 18, Issue 4 https://doi.org/10.1007/BF00020010	journal	February 1992
Evaluation of Selectable Markers for Obtaining Stable Transformants in the Gramineae Hauptmann, R. M.; Vasil, V.; Ozias-Akins, P. Plant Physiology, Vol. 86, Issue 2 https://doi.org/10.1104/pp.86.2.602	journal	February 1988
Selectable marker genes in transgenic plants: applications, alternatives and biosafety Miki, Brian; McHugh, Sylvia Journal of Biotechnology, Vol. 107, Issue 3, p. 193-232 https://doi.org/10.1016/j.jbiotec.2003.10.011	journal	February 2004
Genetic transformation of Indian bread (T. aestivum) and pasta (T. durum) wheat by particle bombardment of mature embryo-derived calli Patnaik, Debasis; Khurana, Paramjit BMC Plant Biology, Vol. 3, Issue 1, p. 5 https://doi.org/10.1186/1471-2229-3-5	journal	January 2003
Agrobacterium tumefaciens-mediated genetic transformation of rye (Secale cereale L.) Popelka, Juan Carlos; Altpeter, Fredy Molecular Breeding, Vol. 11, Issue 3, p. 203-211 https://doi.org/10.1023/A:1022876318276	journal	January 2003
Cell wall composition and digestibility alterations in Brachypodium distachyon achieved through reduced expression of the UDP-arabinopyranose mutase Rancour, David M.; Hatfield, Ronald D.; Marita, Jane M. Frontiers in Plant Science, Vol. 6 https://doi.org/10.3389/fpls.2015.00446	journal	June 2015
Selectable Markers and Reporter Genes: A Well Furnished Toolbox for Plant Science and Genetic Engineering Rosellini, Daniele Critical Reviews in Plant Sciences, Vol. 31, Issue 5 https://doi.org/10.1080/07352689.2012.683373	journal	September 2012
Brachypodium sylvaticum, a Model for Perennial Grasses: Transformation and Inbred Line Development Steinwand, Michael A.; Young, Hugh A.; Bragg, Jennifer N. PLoS ONE, Vol. 8, Issue 9 https://doi.org/10.1371/journal.pone.0075180	journal	September 2013
Advances in selectable marker genes for plant transformation Sundar, Isaac Kirubakaran; Sakthivel, Natarajan Journal of Plant Physiology, Vol. 165, Issue 16 https://doi.org/10.1016/j.jplph.2008.08.002	journal	November 2008
Agrobacterium-mediated transformation of the temperate grass Brachypodium distachyon (genotype Bd21) for T-DNA insertional mutagenesis Vain, Philippe; Worland, Barbara; Thole, Vera Plant Biotechnology Journal, Vol. 6, Issue 3 https://doi.org/10.1111/j.1467-7652.2007.00308.x	journal	April 2008
High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3 Vogel, John; Hill, Theresa Plant Cell Reports, Vol. 27, Issue 3 https://doi.org/10.1007/s00299-007-0472-y	journal	November 2007
Resistance to hygromycin B: A new marker for plant transformation studies Waldron, C.; Murphy, E. B.; Roberts, J. L. Plant Molecular Biology, Vol. 5, Issue 2 https://doi.org/10.1007/BF00020092	journal	January 1985
Selective agents and marker genes for use in transformation of monocotyledonous plants Wilmink, A.; Dons, J. J. M. Plant Molecular Biology Reporter, Vol. 11, Issue 2 https://doi.org/10.1007/bf02670474	journal	June 1993
Isolation and characterization of two tightly linked catalase genes from castor bean that are differentially regulated Suzuki, Masaharu; Ario, Takeshi; Hattori, Tsukaho Plant Molecular Biology, Vol. 25, Issue 3 https://doi.org/10.1007/bf00043878	journal	June 1994
Use of paromomycin as a selective agent for oat transformation Torbert, Kimberly A.; Rines, Howard W.; Somers, David A. Plant Cell Reports, Vol. 14, Issue 10 https://doi.org/10.1007/bf00232728	journal	July 1995
Rapid and reproducible Agrobacterium-mediated transformation of sorghum Howe, Arlene; Sato, Shirley; Dweikat, Ismail Plant Cell Reports, Vol. 25, Issue 8 https://doi.org/10.1007/s00299-005-0081-6	journal	March 2006
The OsGEX2 Gene Promoter Confers Sperm Cell Expression in Transgenic Rice Cook, Meridith; Thilmony, Roger Plant Molecular Biology Reporter, Vol. 30, Issue 5 https://doi.org/10.1007/s11105-012-0429-3	journal	March 2012
pORE: a modular binary vector series suited for both monocot and dicot plant transformation Coutu, Catherine; Brandle, James; Brown, Dan Transgenic Research, Vol. 16, Issue 6 https://doi.org/10.1007/s11248-007-9066-2	journal	February 2007
Effect of promoter driving selectable marker on corn transformation Shiva Prakash, N.; Prasad, V.; Chidambram, Thillai P. Transgenic Research, Vol. 17, Issue 4 https://doi.org/10.1007/s11248-007-9149-0	journal	October 2007
Use of a transient expression assay for the optimization of direct gene transfer into tobacco mesophyll protoplasts by electroporation Guerche, Philippe; Bellini, Catherine; Le Moullec, Jean-Michel Biochimie, Vol. 69, Issue 6-7 https://doi.org/10.1016/0300-9084(87)90181-7	journal	June 1987
Structure, expression and promoter activity of two polyubiquitin genes from rice (Oryza sativa L.) Wang, Jianlin; Jiang, Junda; Oard, James H. Plant Science, Vol. 156, Issue 2 https://doi.org/10.1016/s0168-9452(00)00255-7	journal	July 2000
Rapid production of transgenic turfgrass (Festuca rubra L.) plants Altpeter, Fredy; Xu, Jianping Journal of Plant Physiology, Vol. 157, Issue 4 https://doi.org/10.1016/s0176-1617(00)80029-2	journal	October 2000
Aminoglycoside Antibiotics Inhibit Shoot Regeneration from Siberian Elm Leaf Explants Kapaun, James A.; Cheng, Zong-Ming HortScience, Vol. 34, Issue 4 https://doi.org/10.21273/hortsci.34.4.727	journal	July 1999

Cited By (5)

Accurate measurement of transgene copy number in crop plants using droplet digital PCR Collier, Ray; Dasgupta, Kasturi; Xing, Yan-Ping The Plant Journal, Vol. 90, Issue 5 https://doi.org/10.1111/tpj.13517	journal	April 2017
A versatile and robust Agrobacterium-based gene stacking system generates high-quality transgenic Arabidopsis plants Collier, Ray; Thomson, James G.; Thilmony, Roger The Plant Journal, Vol. 95, Issue 4 https://doi.org/10.1111/tpj.13992	journal	July 2018
In Vitro Tissue Culture in Brachypodium: Applications and Challenges Betekhtin, Alexander; Hus, Karolina; Rojek-Jelonek, Magdalena International Journal of Molecular Sciences, Vol. 21, Issue 3 https://doi.org/10.3390/ijms21031037	journal	February 2020
Draft Genome Sequence of Agrobacterium rhizogenes Strain NCPPB2659 Valdes Franco, Jose A.; Collier, Ray; Wang, Yi Genome Announcements, Vol. 4, Issue 4 https://doi.org/10.1128/genomea.00746-16	journal	August 2016
Optimization of Mature Embryo-Based Tissue Culture and Agrobacterium-Mediated Transformation in Model Grass Brachypodium distachyon International Journal of Molecular Sciences, Vol. 20, Issue 21 https://doi.org/10.3390/ijms20215448	journal	October 2019

Similar Records

Agrobacterium ‐Mediated Transformation of Brachypodium distachyon

Journal Article · Tue Mar 12 00:00:00 EDT 2019 · Current Protocols in Plant Biology · OSTI ID:1282007

Chen, Fengjuan; Liu, Qi; P. Vogel, John; +1 more

Using the Model Perennial Grass Brachypodium sylvaticum to Engineer Resistance to Multiple Abiotic Stresses

Conference · Fri Mar 20 00:00:00 EDT 2015 · OSTI ID:1282007

Gordon, Sean; Reguera, Maria; Sade, Nir; +5 more

Brachypodium sylvaticum, a Model for Perennial Grasses: Transformation and Inbred Line Development

Journal Article · Fri Sep 20 00:00:00 EDT 2013 · PLoS ONE · OSTI ID:1282007

Steinwand, Michael A.; Young, Hugh A.; Bragg, Jennifer N.; +2 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
transformation
Brachypodium
Agrobacterium rhizogenes
paromomycin
tissue culture
callus
selectable marker genes
mediated transformation
plant transformation
particle bombardment
polyubiquitin genes
promoter activity
expression
l.
electroporation
protoplasts

Title: Use of Agrobacterium rhizogenes strain 18r12v and paromomycin selection for transformation of Brachypodium distachyon and Brachypodium sylvaticum

Citation Formats

References (28)

Cited By (5)

Similar Records

Related Subjects