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This content will become publicly available on December 1, 2016

Title: Nanoporous aluminum oxide membranes coated with atomic layer deposition-grown titanium dioxide for biomedical applications: An in vitro evaluation

The surface topographies of nanoporous anodic aluminum oxide (AAO) and titanium dioxide (TiO2) membranes have been shown to modulate cell response in orthopedic and skin wound repair applications. In this study, we: (1) demonstrate an improved atomic layer deposition (ALD) method for coating the porous structures of 20, 100, and 200 nm pore diameter AAO with nanometer-thick layers of TiO2 and (2) evaluate the effects of uncoated AAO and TiO2-coated AAO on cellular responses. The TiO2 coatings were deposited on the AAO membranes without compromising the openings of the nanoscale pores. The 20 nm TiO2-coated membranes showed the highest amount of initial protein adsorption via the micro bicinchoninic acid (micro-BOA) assay; all of the TiO2-coated membranes showed slightly higher protein adsorption than the uncoated control materials. Cell viability, proliferation, and inflammatory responses on the TiO2-coated AAO membranes showed no adverse outcomes. For all of the tested surfaces, normal increases in proliferation (DNA content) of L929 fibroblasts were observed over from 4 hours to 72 hours. No increases in TNF-alpha production were seen in RAW 264.7 macrophages grown on TiO2-coated AAO membranes compared to uncoated AAO membranes and tissue culture polystyrene (TOPS) surfaces. Both uncoated AAO membranes and TiO2-coated AAO membranesmore » showed no significant effects on cell growth and inflammatory responses. In conclusion, the results suggest that TiO2-coated AAO may serve as a reasonable prototype material for the development of nanostructured wound repair devices and orthopedic implants.« less
 [1] ;  [2] ;  [1] ;  [1] ;  [2] ;  [1] ;  [3] ;  [4]
  1. U.S. Food and Drug Administration, Silver Spring, MD (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. UNC/NCSU Joint Dept. of Biomedical Engineering, Raleigh, NC (United States)
  4. U.S. Food and Drug Administration, Silver Spring, MD (United States); UNC/NCSU Joint Dept. of Biomedical Engineering, Raleigh, NC (United States)
Publication Date:
OSTI Identifier:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Journal of Biomedical Nanotechnology
Additional Journal Information:
Journal Volume: 11; Journal Issue: 12; Journal ID: ISSN 1550-7033
American Scientific Publishers
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
Sponsoring Org:
Country of Publication:
United States
36 MATERIALS SCIENCE; 59 BASIC BIOLOGICAL SCIENCES anodized aluminum oxide (AAO); titanium dioxide (TiO2); atomic layer deposition (ALD); nanoporous membrane; protein adsorption; biocompatibility; cytotoxicity TNF-alpha