Matrix recrystallization for MALDI-MS imaging of maize lipids at high-spatial resolution

Duenas, Maria Emilia; Carlucci, Laura; Lee, Young Jin

doi:10.1007/s13361-016-1422-0

Title: Matrix recrystallization for MALDI-MS imaging of maize lipids at high-spatial resolution

Journal Article · Mon Jun 27 00:00:00 EDT 2016 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/s13361-016-1422-0· OSTI ID:1278749

Duenas, Maria Emilia ^[1]; Carlucci, Laura ^[2]; Lee, Young Jin ^[1]

Ames Lab. and Iowa State Univ., Ames, IA (United States)
Ames Lab., Ames, IA (United States)

Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos and leaves. A systematic study was performed varying solvent and incubation time. During this study, unexpected side reactions were found when methanol was used as a recrystallization solvent, resulting in the formation of a methyl ester of phosphatidic acid. Furthermore, using an optimum recrystallization condition with isopropanol, there is no apparent delocalization demonstrated with a transmission electron microscopy (TEM) pattern and maize leaf images obtained at 10 μm spatial resolution.

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Research Organization:: Ames Laboratory (AMES), Ames, IA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-07CH11358

OSTI ID:: 1278749

Report Number(s):: IS-J-8999; PII: 1422

Journal Information:: Journal of the American Society for Mass Spectrometry, Journal Name: Journal of the American Society for Mass Spectrometry; ISSN 1044-0305

Publisher:: American Society for Mass SpectrometryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 28 works

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References (13)

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Spatial analysis of lipid metabolites and expressed genes reveals tissue-specific heterogeneity of lipid metabolism in high- and low-oil Brassica napus L. seeds Lu, Shaoping; Sturtevant, Drew; Aziz, Mina The Plant Journal, Vol. 94, Issue 6 https://doi.org/10.1111/tpj.13959	journal	June 2018
Spatial distribution mapping of molecules in the grains of different rice landraces, using desorption electrospray ionization mass spectrometry Suganya, Arunan; Deb, Debal; Pradeep, Thalappil Rapid Communications in Mass Spectrometry, Vol. 33, Issue 7 https://doi.org/10.1002/rcm.8397	journal	March 2019
Recent advances in matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) for in situ analysis of endogenous molecules in plants Qin, Liang; Zhang, Yawen; Liu, Yaqin Phytochemical Analysis, Vol. 29, Issue 4 https://doi.org/10.1002/pca.2759	journal	April 2018
Toward Mass Spectrometry Imaging in the Metabolomics Scale: Increasing Metabolic Coverage Through Multiple On-Tissue Chemical Modifications Dueñas, Maria Emilia; Larson, Evan A.; Lee, Young Jin Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.00860	journal	July 2019

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
mass spectrometry imaging
MALDI
matrix
recrystallization
maize
lipids
high spatial resolution

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