Phosphorylation of spore coat proteins by a family of atypical protein kinases

Nguyen, Kim B.; Sreelatha, Anju; Durrant, Eric S.; Lopez-Garrido, Javier; Muszewska, Anna; Dudkiewicz, Małgorzata; Grynberg, Marcin; Yee, Samantha; Pogliano, Kit; Tomchick, Diana R.; Pawłowski, Krzysztof; Dixon, Jack E.; Tagliabracci, Vincent S.

doi:10.1073/pnas.1605917113

Title: Phosphorylation of spore coat proteins by a family of atypical protein kinases

Journal Article · Mon May 16 00:00:00 EDT 2016 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1605917113· OSTI ID:1274751

Nguyen, Kim B. ^[1]; Sreelatha, Anju ^[2]; Durrant, Eric S. ^[1]; Lopez-Garrido, Javier ^[3]; Muszewska, Anna ^[4]; Dudkiewicz, Małgorzata ^[5]; Grynberg, Marcin ^[6]; Yee, Samantha ^[2]; Pogliano, Kit ^[3]; Tomchick, Diana R. ^[7]; Pawłowski, Krzysztof ^[5]; Dixon, Jack E. ^[8]; Tagliabracci, Vincent S. ^[2]

Univ. of California, San Diego, CA (United States). Dept. of Pharmacology
Univ. of Texas Southwestern Medical Center, Dallas, TX (United States). Dept. of Molecular Biology
Univ. of California, San Diego, CA (United States). Division of Biological Sciences
Polish Academy of Sciences (PAS), Warsaw (Poland). Inst. of Biochemistry and Biophysics. Dept. of Microbial Biochemistry
Warsaw Univ. of Life Sciences (Poland). Dept. of Experimental Design and Bioinformatics
Polish Academy of Sciences (PAS), Warsaw (Poland). Inst. of Biochemistry and Biophysics. Dept. of Biophysics
Univ. of Texas Southwestern Medical Center, Dallas, TX (United States). Dept. of Biophysics and Biochemistry
Univ. of California, San Diego, CA (United States). Dept. of Pharmacology. Dept. of Cellular and Molecular Medicine. Dept. of Chemistry and Biochemistry

The modification of proteins by phosphorylation occurs in all life forms and is catalyzed by a large superfamily of enzymes known as protein kinases. We recently discovered a family of secretory pathway kinases that phosphorylate extracellular proteins. One member, family with sequence similarity 20C (Fam20C), is the physiological Golgi casein kinase. While examining distantly related protein sequences, we observed low levels of identity between the spore coat protein H (CotH), and the Fam20C-related secretory pathway kinases. CotH is a component of the spore in many bacterial and eukaryotic species, and is required for efficient germination of spores in Bacillus subtilis; however, the mechanism by which CotH affects germination is unclear. In this paper, we show that CotH is a protein kinase. The crystal structure of CotH reveals an atypical protein kinase-like fold with a unique mode of ATP binding. Examination of the genes neighboring cotH in B. subtilis led us to identify two spore coat proteins, CotB and CotG, as CotH substrates. Furthermore, we show that CotH-dependent phosphorylation of CotB and CotG is required for the efficient germination of B. subtilis spores. Finally and collectively, our results define a family of atypical protein kinases and reveal an unexpected role for protein phosphorylation in spore biology.

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Research Organization:: Univ. of California, San Diego, CA (United States); Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Inst. of Health (NIH) (United States); Polish National Science Centre (Poland)

Contributing Organization:: Polish Academy of Sciences (PAS), Warsaw (Poland); Warsaw Univ. of Life Sciences (Poland)

Grant/Contract Number:: AC02-06CH11357; DK018849; DK018024; R00DK099254; GM57045; 2012/05/B/NZ3/00413; 2012/07/D/NZ2/04286; RR150033

OSTI ID:: 1274751

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 25; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 42 works

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Protein post-translational modifications in bacteria Macek, Boris; Forchhammer, Karl; Hardouin, Julie Nature Reviews Microbiology, Vol. 17, Issue 11 https://doi.org/10.1038/s41579-019-0243-0	journal	September 2019

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