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Title: A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling

Journal Article · · BMC Genomics
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In the last 5 years, the rapid pace of innovations and improvements in sequencing technologies has completely changed the landscape of metagenomic and metagenetic experiments. Therefore, it is critical to benchmark the various methodologies for interrogating the composition of microbial communities, so that we can assess their strengths and limitations. Here, the most common phylogenetic marker for microbial community diversity studies is the 16S ribosomal RNA gene and in the last 10 years the field has moved from sequencing a small number of amplicons and samples to more complex studies where thousands of samples and multiple different gene regions are interrogated.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Laboratory Directed Research and Development (LDRD) Program
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1618593
Alternate ID(s):
OSTI ID: 1265994
Journal Information:
BMC Genomics, Journal Name: BMC Genomics Vol. 17 Journal Issue: 1; ISSN 1471-2164
Publisher:
Springer Science + Business MediaCopyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 237 works
Citation information provided by
Web of Science

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