In vivo epidermal migration requires focal adhesion targeting of ACF7

Yue, Jiping; Zhang, Yao; Liang, Wenguang G.; Gou, Xuewen; Lee, Philbert; Liu, Han; Lyu, Wanqing; Tang, Wei -Jen; Chen, Shao -Yu; Yang, Feng; Liang, Hong; Wu, Xiaoyang

doi:10.1038/ncomms11692

Title: In vivo epidermal migration requires focal adhesion targeting of ACF7

Journal Article · Tue May 24 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms11692· OSTI ID:1261624

Yue, Jiping ^[1]; Zhang, Yao ^[2]; Liang, Wenguang G. ^[1]; Gou, Xuewen ^[1]; Lee, Philbert ^[1]; Liu, Han ^[1]; Lyu, Wanqing ^[1]; Tang, Wei -Jen ^[1]; Chen, Shao -Yu ^[3]; Yang, Feng ^[2]; Liang, Hong ^[2]; Wu, Xiaoyang ^[1]

The Univ. of Chicago, Chicago, IL (United States)
Guanxi Normal Univ., Guilin (China)
Univ. of Louisville, Louisville, KY (United States)

Turnover of focal adhesions allows cell retraction, which is essential for cell migration. The mammalian spectraplakin protein, ACF7 (Actin-Crosslinking Factor 7), promotes focal adhesion dynamics by targeting of microtubule plus ends towards focal adhesions. However, it remains unclear how the activity of ACF7 is regulated spatiotemporally to achieve focal adhesion-specific guidance of microtubule. To explore the potential mechanisms, we resolve the crystal structure of ACF7's NT (amino-terminal) domain, which mediates F-actin interactions. Structural analysis leads to identification of a key tyrosine residue at the calponin homology (CH) domain of ACF7, whose phosphorylation by Src/FAK (focal adhesion kinase) complex is essential for F-actin binding of ACF7. Using skin epidermis as a model system, we further demonstrate that the phosphorylation of ACF7 plays an indispensable role in focal adhesion dynamics and epidermal migration in vitro and in vivo. Altogether, our findings provide critical insights into the molecular mechanisms underlying coordinated cytoskeletal dynamics during cell movement.

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Research Organization:: Univ. of Chicago, IL (United States). Ben May Dept. for Cancer Research

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Cancer Institute (NCI); University of Chicago; National Institutes of Health (NIH); American Cancer Society; V foundation; Natural Science Foundation of China; Natural Science Foundation of Guangxi

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1261624

Journal Information:: Nature Communications, Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 41 works

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Title: In vivo epidermal migration requires focal adhesion targeting of ACF7

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