Serial femtosecond crystallography of soluble proteins in lipidic cubic phase

Fromme, Raimund; Ishchenko, Andrii; Metz, Markus; Chowdhury, Shatabdi Roy; Basu, Shibom; Boutet, Sébastien; Fromme, Petra; White, Thomas A.; Barty, Anton; Spence, John C.  H.; Weierstall, Uwe; Liu, Wei; Cherezov, Vadim

doi:10.1107/S2052252515013160

Title: Serial femtosecond crystallography of soluble proteins in lipidic cubic phase

Journal Article · Tue Aug 04 00:00:00 EDT 2015 · IUCrJ

DOI:https://doi.org/10.1107/S2052252515013160· OSTI ID:1260560

Fromme, Raimund; Ishchenko, Andrii; Metz, Markus; Chowdhury, Shatabdi Roy; Basu, Shibom; Boutet, Sébastien; Fromme, Petra; White, Thomas A.; Barty, Anton; Spence, John C. H.; Weierstall, Uwe; Liu, Wei; Cherezov, Vadim

Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP–SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP–SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-76SF0051

OSTI ID:: 1260560

Journal Information:: IUCrJ, Vol. 2, Issue 5; ISSN 2052-2525

Publisher:: International Union of Crystallography

Country of Publication:: United States

Language:: English

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