Capture of a third Mg2+ is essential for catalyzing DNA synthesis

Gao, Yang; Yang, Wei

doi:10.1126/science.aad9633

Title: Capture of a third Mg²⁺ is essential for catalyzing DNA synthesis

Journal Article · Thu Jun 09 00:00:00 EDT 2016 · Science

DOI:https://doi.org/10.1126/science.aad9633· OSTI ID:1259887

Gao, Yang ^[1]; Yang, Wei ^[1]

National Institutes of Health, Bethesda, MD (United States)

DNA polymerase is an enzyme that uses existing DNA as a template to build new DNA by adding new nucleotides to the end of the newly forming daughter strand. The enzyme mechanism that catalyzes formation of a phosphodiester bond is known to require two Mg²⁺ ions, and recent crystal structures have shown that a third metal ion is present after bond formation. Gao et al. used time-resolved crystallography to visualize bond formation. The enzyme-substrate complex captures a third cation before bond formation occurs, and DNA synthesis cannot occur without the third metal ion. Binding of this metal ion requires thermal motion of the enzyme-substrate complex, so that catalysis is achieved by acquiring a transient cofactor.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: DK036146–08

OSTI ID:: 1259887

Journal Information:: Science, Vol. 352, Issue 6291; ISSN 0036-8075

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 124 works

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