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Title: Increasing cell culture population doublings for long-term growth of finite life span human cell cultures

Patent ·
OSTI ID:1259682
;

Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
9,376,662
Application Number:
14/627,888
OSTI ID:
1259682
Resource Relation:
Patent File Date: 2015 Feb 20
Country of Publication:
United States
Language:
English

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Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract. journal September 1984
Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype journal January 1999
Telomerase protects developing neurons against DNA damage-induced cell death journal November 2001
Absence of cancer–associated changes in human fibroblasts immortalized with telomerase journal January 1999
Telomerase reverse transcriptase promotes cardiac muscle cell proliferation, hypertrophy, and survival journal August 2001
Peripherally Administered Angiotensin II AT 1 Receptor Antagonists Are Anti-stress Compounds in Vivo journal December 2008
Telomerase modulates expression of growth-controlling genes and enhances cell proliferation journal April 2003
Expression of the telomerase catalytic subunit, hTERT, induces resistance to transforming growth factor β growth inhibition in p16INK4A(−) human mammary epithelial cells journal April 2001
Long-term culture of adult murine epidermal keratinocytes journal December 2005
Enhanced growth medium and method for culturing human mammary epithelial cells patent December 1983
Continuous human cell lines and method of making same patent February 1989
Defined systems for epithelial cell culture and use thereof patent February 2004

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