Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius
- grid.417824.c 0000000100207392 Biological and Chemical Processing Department Idaho National Laboratory Idaho Falls ID USA
- grid.451303.0 0000000122183491 Energy and Environment Directorate Pacific Northwest National Laboratory Richland WA USA
Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the concentration of the phenolic compounds: phenol, ferulic acid, ρ-coumaric acid and sinapinic acid during growth conditions. The extent and rate of the removal of these compounds were significantly increased by the presence of micro-molar copper concentrations, suggesting activity by copper oxidases that have been identified in the genome of A. acidocaldarius. Substrate removal kinetics was first order for phenol, ferulic acid, ρ-coumaric acid and sinapinic acid in the presence of 50 μM copper sulfate. In addition, laccase enzyme assays of cellular protein fractions suggested significant activity on a lignin analog between the temperatures of 45 and 90 °C. As a result, this work shows the potential for A. acidocaldarius to degrade phenolic compounds, demonstrating potential relevance to biofuel production and other industrial processes.
- Research Organization:
- Idaho National Laboratory (INL), Idaho Falls, ID (United States)
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- AC07-05ID14517
- OSTI ID:
- 1773514
- Alternate ID(s):
- OSTI ID: 1259505
- Report Number(s):
- INL/JOU-13-30180
- Journal Information:
- Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Vol. 43 Journal Issue: 1; ISSN 1367-5435
- Publisher:
- Oxford University PressCopyright Statement
- Country of Publication:
- Germany
- Language:
- English
Web of Science
Stable-Isotope Probing-Enabled Cultivation of the Indigenous Bacterium Ralstonia sp. Strain M1, Capable of Degrading Phenanthrene and Biphenyl in Industrial Wastewater
|
journal | May 2019 |
Similar Records
Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius
Thermophilic and thermoacidophilic biopolymer degrading genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods