Structure and membrane remodeling activity of ESCRT-III helical polymers

McCullough, J.; Clippinger, A. K.; Talledge, N.; Skowyra, M. L.; Saunders, M. G.; Naismith, T. V.; Colf, L. A.; Afonine, P.; Arthur, C.; Sundquist, W. I.; Hanson, P. I.; Frost, A.

doi:10.1126/science.aad8305

Title: Structure and membrane remodeling activity of ESCRT-III helical polymers

Journal Article · Thu Dec 03 00:00:00 EST 2015 · Science

DOI:https://doi.org/10.1126/science.aad8305· OSTI ID:1355856

McCullough, J.; Clippinger, A. K.; Talledge, N.; Skowyra, M. L.; Saunders, M. G.; Naismith, T. V.; Colf, L. A.; Afonine, P.; Arthur, C.; Sundquist, W. I.; Hanson, P. I.; Frost, A.

The endosomal sorting complexes required for transport (ESCRT) proteins mediate fundamental membrane remodeling events that require stabilizing negative membrane curvature. These include endosomal intralumenal vesicle formation, HIV budding, nuclear envelope closure, and cytokinetic abscission. ESCRT-III subunits perform key roles in these processes by changing conformation and polymerizing into membrane-remodeling filaments. Here, we report the 4 angstrom resolution cryogenic electron microscopy reconstruction of a one-start, double-stranded helical copolymer composed of two different human ESCRT-III subunits, charged multivesicular body protein 1B (CHMP1B) and increased sodium tolerance 1 (IST1). The inner strand comprises "open" CHMP1B subunits that interlock in an elaborate domain-swapped architecture and is encircled by an outer strand of "closed" IST1 subunits. Unlike other ESCRT-III proteins, CHMP1B and IST1 polymers form external coats on positively curved membranes in vitro and in vivo. Our analysis suggests how common ESCRT-III filament architectures could stabilize different degrees and directions of membrane curvature.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231; DGE-1143954; 2P50GM082545-06; R01GM112080; R01AI051174; 1DP2GM110772-01; 1P01 GM063210; R01GM076686; R01NS050717

OSTI ID:: 1355856

Alternate ID(s):: OSTI ID: 1257996; OSTI ID: 1378702

Journal Information:: Science, Journal Name: Science Vol. 350 Journal Issue: 6267; ISSN 0036-8075

Publisher:: American Association for the Advancement of Science (AAAS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 163 works

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