NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability

Parplys, Ann C.; Zhao, Weixing; Sharma, Neelam; Groesser, Torsten; Liang, Fengshan; Maranon, David G.; Leung, Stanley G.; Grundt, Kirsten; Dray, Eloïse; Idate, Rupa; Østvold, Anne Carine; Schild, David; Sung, Patrick; Wiese, Claudia

doi:10.1093/nar/gkv859

Title: NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability

Journal Article · Mon Aug 31 00:00:00 EDT 2015 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkv859· OSTI ID:1257378

Parplys, Ann C. ^[1]; Zhao, Weixing ^[2]; Sharma, Neelam ^[3]; Groesser, Torsten ^[1]; Liang, Fengshan ^[2]; Maranon, David G. ^[3]; Leung, Stanley G. ^[1]; Grundt, Kirsten ^[4]; Dray, Eloïse ^[2]; Idate, Rupa ^[3]; Østvold, Anne Carine ^[4]; Schild, David ^[1]; Sung, Patrick ^[2]; Wiese, Claudia ^[5]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Yale Univ. School of Medicine, New Haven, CT (United States)
Colorado State Univ., Fort Collins, CO (United States)
Univ. of Oslo (Norway)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Colorado State Univ., Fort Collins, CO (United States)

NUCKS1 (nuclear casein kinase and cyclin-dependent kinase substrate 1) is a 27 kD chromosomal, vertebrate-specific protein, for which limited functional data exist. Here, we demonstrate that NUCKS1 shares extensive sequence homology with RAD51AP1 (RAD51 associated protein 1), suggesting that these two proteins are paralogs. Similar to the phenotypic effects of RAD51AP1 knockdown, we find that depletion of NUCKS1 in human cells impairs DNA repair by homologous recombination (HR) and chromosome stability. Depletion of NUCKS1 also results in greatly increased cellular sensitivity to mitomycin C (MMC), and in increased levels of spontaneous and MMC-induced chromatid breaks. NUCKS1 is critical to maintaining wild type HR capacity, and, as observed for a number of proteins involved in the HR pathway, functional loss of NUCKS1 leads to a slow down in DNA replication fork progression with a concomitant increase in the utilization of new replication origins. Interestingly, recombinant NUCKS1 shares the same DNA binding preference as RAD51AP1, but binds to DNA with reduced affinity when compared to RAD51AP1. Finally, our results show that NUCKS1 is a chromatin-associated protein with a role in the DNA damage response and in HR, a DNA repair pathway critical for tumor suppression.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1257378

Journal Information:: Nucleic Acids Research, Vol. 43, Issue 20; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 40 works

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References (83)

DNA Repair Deficiency and Neurodegeneration Katyal, Sachin; McKinnon, Peter J. Cell Cycle, Vol. 6, Issue 19 https://doi.org/10.4161/cc.6.19.4757	journal	October 2007
DNA strand breaks, neurodegeneration and aging in the brain Katyal, Sachin; McKinnon, Peter J. Mechanisms of Ageing and Development, Vol. 129, Issue 7-8 https://doi.org/10.1016/j.mad.2008.03.008	journal	July 2008
Ability to repair DNA double-strand breaks related to cancer susceptibility and radiosensitivity Sakata, Koh-ichi; Someya, Masanori; Matsumoto, Yoshihisa Radiation Medicine, Vol. 25, Issue 9 https://doi.org/10.1007/s11604-007-0161-3	journal	November 2007
V(D)J Recombination Deficiencies de Villartay, Jean-Pierre Advances in Experimental Medicine and Biology https://doi.org/10.1007/978-1-4419-0296-2_4	book	January 2009
Homology-Directed Repair is Required for the Development of Radioresistance during S Phase: Interplay between Double-Strand Break Repair and Checkpoint Response Tamulevicius, Peter; Wang, Minli; Iliakis, George Radiation Research, Vol. 167, Issue 1 https://doi.org/10.1667/RR0751.1	journal	January 2007
Promotion of Homologous Recombination and Genomic Stability by RAD51AP1 via RAD51 Recombinase Enhancement Wiese, Claudia; Dray, Eloïse; Groesser, Torsten Molecular Cell, Vol. 28, Issue 3 https://doi.org/10.1016/j.molcel.2007.08.027	journal	November 2007
Molecular basis for enhancement of the meiotic DMC1 recombinase by RAD51 associated protein 1 (RAD51AP1) Dray, Eloïse; Dunlop, Myun Hwa; Kauppi, Liisa Proceedings of the National Academy of Sciences, Vol. 108, Issue 9 https://doi.org/10.1073/pnas.1016454108	journal	February 2011
RAD51-associated Protein 1 (RAD51AP1) Interacts with the Meiotic Recombinase DMC1 through a Conserved Motif Dunlop, Myun Hwa; Dray, Eloïse; Zhao, Weixing Journal of Biological Chemistry, Vol. 286, Issue 43 https://doi.org/10.1074/jbc.M111.290015	journal	September 2011
Mechanistic Insights into RAD51-associated Protein 1 (RAD51AP1) Action in Homologous DNA Repair Dunlop, Myun Hwa; Dray, Eloïse; Zhao, Weixing Journal of Biological Chemistry, Vol. 287, Issue 15 https://doi.org/10.1074/jbc.C112.352161	journal	February 2012
RAD51AP1 -deficiency in vertebrate cells impairs DNA replication Parplys, Ann C.; Kratz, Katja; Speed, Michael C. DNA Repair, Vol. 24 https://doi.org/10.1016/j.dnarep.2014.09.007	journal	December 2014
Heterozygous mutations in PALB2 cause DNA replication and damage response defects Nikkilä, Jenni; Christin Parplys, Ann; Pylkäs, Katri Nature Communications, Vol. 4, Issue 1 https://doi.org/10.1038/ncomms3578	journal	October 2013
Homologous Recombination Is a Primary Pathway to Repair DNA Double-Strand Breaks Generated during DNA Rereplication Truong, Lan N.; Li, Yongjiang; Sun, Emily Journal of Biological Chemistry, Vol. 289, Issue 42 https://doi.org/10.1074/jbc.M114.576488	journal	August 2014
Pir51, a Rad51-interacting protein with high expression in aggressive lymphoma, controls mitomycin C sensitivity and prevents chromosomal breaks Henson, Sarah E.; Tsai, Shih-Chang; Malone, Cindy Sue Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 601, Issue 1-2 https://doi.org/10.1016/j.mrfmmm.2006.06.016	journal	October 2006
RAD51AP1 Is a Structure-Specific DNA Binding Protein that Stimulates Joint Molecule Formation during RAD51-Mediated Homologous Recombination Modesti, Mauro; Budzowska, Magda; Baldeyron, Céline Molecular Cell, Vol. 28, Issue 3 https://doi.org/10.1016/j.molcel.2007.08.025	journal	November 2007
Characterisation of the NUCKS gene on human chromosome 1q32.1 and the presence of a homologous gene in different species Grundt, Kirsten; Haga, Ingvild Vågslid; Aleporou-Marinou, Vasiliki Biochemical and Biophysical Research Communications, Vol. 323, Issue 3 https://doi.org/10.1016/j.bbrc.2004.08.153	journal	October 2004
Identification and characterization of two putative nuclear localization signals (NLS) in the DNA-binding protein NUCKS Grundt, Kirsten; Haga, Ingvild V.; Huitfeldt, Henrik S. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1773, Issue 9 https://doi.org/10.1016/j.bbamcr.2007.05.013	journal	September 2007
Constitutive and dynamic phosphorylation and acetylation sites on NUCKS, a hypermodified nuclear protein, studied by quantitative proteomics Wiśniewski, Jacek R.; Zougman, Alexandre; Krüger, Sonja Proteins: Structure, Function, and Bioinformatics, Vol. 73, Issue 3 https://doi.org/10.1002/prot.22104	journal	May 2008
A novel, highly phosphorylated protein, of the high-mobility group type, present in a variety of proliferating and non-proliferating mammalian cells Ostvold, Anne C.; Holtlund, Jostein; Laland, Soren G. European Journal of Biochemistry, Vol. 153, Issue 3 https://doi.org/10.1111/j.1432-1033.1985.tb09325.x	journal	December 1985
Identification and Confirmation of a Module of Coexpressed Genes Thompson, H. G. R. Genome Research, Vol. 12, Issue 10 https://doi.org/10.1101/gr.418402	journal	October 2002
Chromosomal changes in high- and low-invasive mouse lung adenocarcinoma cell strains derived from early passage mouse lung adenocarcinoma cell strains Sargent, Linda M.; Ensell, Mang X.; Ostvold, Anne-Carine Toxicology and Applied Pharmacology, Vol. 233, Issue 1 https://doi.org/10.1016/j.taap.2008.01.031	journal	November 2008
NUCKS overexpression in breast cancer Drosos, Yiannis; Kouloukoussa, Mirsini; Østvold, Anne Cancer Cell International, Vol. 9, Issue 1 https://doi.org/10.1186/1475-2867-9-19	journal	January 2009
Identification of NUCKS1 as a colorectal cancer prognostic marker through integrated expression and copy number analysis Kikuchi, Akifumi; Ishikawa, Toshiaki; Mogushi, Kaoru International Journal of Cancer, Vol. 132, Issue 10 https://doi.org/10.1002/ijc.27911	journal	November 2012
Altered expression of miR-24, miR-126 and miR-365 does not affect viability of childhood TCF3-rearranged leukemia cells Akbari Moqadam, F.; Boer, J. M.; Lange-Turenhout, E. A. M. Leukemia, Vol. 28, Issue 5 https://doi.org/10.1038/leu.2013.308	journal	October 2013
Combined evaluation of the expression of NUCKS and Ki-67 proteins as independent prognostic factors for patients with gastric adenocarcinoma Yang, Ming; Wang, Xiaoxia; Zhao, Qi Tumor Biology, Vol. 35, Issue 8 https://doi.org/10.1007/s13277-014-1880-6	journal	May 2014
The comparison of nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) with Ki67 proliferation marker expression in common skin tumors Zduniak, Krzysztof; Agrawal, Siddarth; Symonowicz, Krzysztof Polish Journal of Pathology, Vol. 1 https://doi.org/10.5114/pjp.2014.42669	journal	January 2014
NUCKS Is a Positive Transcriptional Regulator of Insulin Signaling Qiu, Beiying; Shi, Xiaohe; Wong, Ee Tsin Cell Reports, Vol. 7, Issue 6 https://doi.org/10.1016/j.celrep.2014.05.030	journal	June 2014
NUCKS: a potential biomarker in cancer and metabolic disease Qiu, Beiying; Han, Weiping; Tergaonkar, Vinay Clinical Science, Vol. 128, Issue 10 https://doi.org/10.1042/CS20140656	journal	March 2015
ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage Matsuoka, S.; Ballif, B. A.; Smogorzewska, A. Science, Vol. 316, Issue 5828 https://doi.org/10.1126/science.1140321	journal	May 2007
ATM-Dependent and -Independent Dynamics of the Nuclear Phosphoproteome After DNA Damage Bensimon, A.; Schmidt, A.; Ziv, Y. Science Signaling, Vol. 3, Issue 151 https://doi.org/10.1126/scisignal.2001034	journal	December 2010
Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions Gorgoulis, Vassilis G.; Vassiliou, Leandros-Vassilios F.; Karakaidos, Panagiotis Nature, Vol. 434, Issue 7035 https://doi.org/10.1038/nature03485	journal	April 2005
An Oncogene-Induced DNA Damage Model for Cancer Development Halazonetis, T. D.; Gorgoulis, V. G.; Bartek, J. Science, Vol. 319, Issue 5868 https://doi.org/10.1126/science.1140735	journal	March 2008
Fibronectin expression increases during in vitro cellular senescence: Correlation with increased cell area Kumazaki, Tsutomu; Robetorye, Ryan S.; Robetorye, Susan C. Experimental Cell Research, Vol. 195, Issue 1 https://doi.org/10.1016/0014-4827(91)90494-F	journal	July 1991
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair Nakanishi, K.; Yang, Y. -G.; Pierce, A. J. Proceedings of the National Academy of Sciences, Vol. 102, Issue 4 https://doi.org/10.1073/pnas.0407796102	journal	January 2005
Control of BRCA2 Cellular and Clinical Functions by a Nuclear Partner, PALB2 Xia, Bing; Sheng, Qing; Nakanishi, Koji Molecular Cell, Vol. 22, Issue 6 https://doi.org/10.1016/j.molcel.2006.05.022	journal	June 2006
Homology-directed Fanconi anemia pathway cross-link repair is dependent on DNA replication Nakanishi, Koji; Cavallo, Francesca; Perrouault, Loïc Nature Structural & Molecular Biology, Vol. 18, Issue 4 https://doi.org/10.1038/nsmb.2029	journal	March 2011
Homologous Recombination Contributes to the Repair of DNA Double-Strand Breaks Induced by High-Energy Iron Ions Zafar, Faria; Seidler, Sara B.; Kronenberg, Amy Radiation Research, Vol. 173, Issue 1 https://doi.org/10.1667/RR1910.1	journal	January 2010
Double-strand break repair by interchromosomal recombination: suppression of chromosomal translocations Richardson, C.; Moynahan, M. E.; Jasin, M. Genes & Development, Vol. 12, Issue 24 https://doi.org/10.1101/gad.12.24.3831	journal	December 1998
Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombination Wiese, C.; Hinz, J. M.; Tebbs, R. S. Nucleic Acids Research, Vol. 34, Issue 9 https://doi.org/10.1093/nar/gkl366	journal	January 2006
Enhancement of RAD51 recombinase activity by the tumor suppressor PALB2 Dray, Eloïse; Etchin, Julia; Wiese, Claudia Nature Structural & Molecular Biology, Vol. 17, Issue 10 https://doi.org/10.1038/nsmb.1916	journal	September 2010
Molecular cloning of a mammalian nuclear phosphoprotein NUCKS, which serves as a substrate for Cdk1 in vivo: Molecular cloning of NUCKS Østvold, Anne C.; Norum, Jens H.; Mathiesen, Siri European Journal of Biochemistry, Vol. 268, Issue 8 https://doi.org/10.1046/j.1432-1327.2001.02120.x	journal	April 2001
Iterative Voting for Inference of Structural Saliency and Characterization of Subcellular Events Parvin, Bahram; Yang, Qing; Han, Ju IEEE Transactions on Image Processing, Vol. 16, Issue 3 https://doi.org/10.1109/TIP.2007.891154	journal	March 2007
Geometric approach to segmentation and protein localization in cell culture assays Raman, S.; Maxwell, C. A.; Barcellos-Hoff, M. H. Journal of Microscopy, Vol. 225, Issue 1 https://doi.org/10.1111/j.1365-2818.2007.01712.x	journal	January 2007
XRCC3 and Rad51 Modulate Replication Fork Progression on Damaged Vertebrate Chromosomes Henry-Mowatt, Judith; Jackson, Dean; Masson, Jean-Yves Molecular Cell, Vol. 11, Issue 4 https://doi.org/10.1016/S1097-2765(03)00132-1	journal	April 2003
Replicon Clusters Are Stable Units of Chromosome Structure: Evidence That Nuclear Organization Contributes to the Efficient Activation and Propagation of S Phase in Human Cells Jackson, Dean A.; Pombo, Ana Journal of Cell Biology, Vol. 140, Issue 6 https://doi.org/10.1083/jcb.140.6.1285	journal	March 1998
Basis for Avid Homologous DNA Strand Exchange by Human Rad51 and RPA Sigurdsson, Stefan; Trujillo, Kelly; Song, BinWei Journal of Biological Chemistry, Vol. 276, Issue 12 https://doi.org/10.1074/jbc.M010011200	journal	December 2000
The coactivators CBP/p300 and the histone chaperone NAP1 promote transcription-independent nucleosome eviction at the HTLV-1 promoter Sharma, N.; Nyborg, J. K. Proceedings of the National Academy of Sciences, Vol. 105, Issue 23 https://doi.org/10.1073/pnas.0800534105	journal	June 2008
RAD51AP2, a novel vertebrate- and meiotic-specific protein, shares a conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51 Kovalenko, Oleg V.; Wiese, Claudia; Schild, David Nucleic Acids Research, Vol. 34, Issue 18 https://doi.org/10.1093/nar/gkl665	journal	September 2006
Cytogenetic characterization of the ionizing radiation-sensitive Chinese hamster mutant irs1 Tucker, James D.; Jones, Nigel J.; Allen, Nancy A. Mutation Research/DNA Repair, Vol. 254, Issue 2 https://doi.org/10.1016/0921-8777(91)90005-A	journal	March 1991
DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation Bakkenist, Christopher J.; Kastan, Michael B. Nature, Vol. 421, Issue 6922, p. 499-506 https://doi.org/10.1038/nature01368	journal	January 2003
Effect of in vivo Histone Hyperacetylation on the State of Chromatin Fibers Krajewski, Wladyslaw A. Journal of Biomolecular Structure and Dynamics, Vol. 16, Issue 5 https://doi.org/10.1080/07391102.1999.10508318	journal	April 1999
Regulation of Homologous Recombination by RNF20-Dependent H2B Ubiquitination Nakamura, Kyosuke; Kato, Akihiro; Kobayashi, Junya Molecular Cell, Vol. 41, Issue 5 https://doi.org/10.1016/j.molcel.2011.02.002	journal	March 2011
Influence of homologous recombinational repair on cell survival and chromosomal aberration induction during the cell cycle in γ-irradiated CHO cells Wilson, Paul F.; Hinz, John M.; Urbin, Salustra S. DNA Repair, Vol. 9, Issue 7 https://doi.org/10.1016/j.dnarep.2010.03.009	journal	July 2010
Snm1B/Apollo mediates replication fork collapse and S Phase checkpoint activation in response to DNA interstrand cross-links Bae, J-B; Mukhopadhyay, S. S.; Liu, L. Oncogene, Vol. 27, Issue 37 https://doi.org/10.1038/onc.2008.139	journal	May 2008
Deletion of BRCA2 exon 27 causes defects in response to both stalled and collapsed replication forks Kim, Tae Moon; Son, Mi Young; Dodds, Sherry Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 766-767 https://doi.org/10.1016/j.mrfmmm.2014.06.003	journal	August 2014
Pathways for mitotic homologous recombination in mammalian cells Helleday, T. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 532, Issue 1-2 https://doi.org/10.1016/j.mrfmmm.2003.08.013	journal	November 2003
Multiple interlinked mechanisms to circumvent DNA replication roadblocks Eppink, Berina; Wyman, Claire; Kanaar, Roland Experimental Cell Research, Vol. 312, Issue 14 https://doi.org/10.1016/j.yexcr.2006.06.011	journal	August 2006
Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells Wilhelm, T.; Magdalou, I.; Barascu, A. Proceedings of the National Academy of Sciences, Vol. 111, Issue 2 https://doi.org/10.1073/pnas.1311520111	journal	December 2013
The Intra-S-Phase Checkpoint Affects both DNA Replication Initiation and Elongation: Single-Cell and -DNA Fiber Analyses Seiler, Jennifer A.; Conti, Chiara; Syed, Ali Molecular and Cellular Biology, Vol. 27, Issue 16 https://doi.org/10.1128/MCB.02278-06	journal	May 2007
A novel nucleic acid-binding protein that interacts with human rad51 recombinase Kovalenko, O. Nucleic Acids Research, Vol. 25, Issue 24 https://doi.org/10.1093/nar/25.24.4946	journal	December 1997
High resolution genomic analysis of sporadic breast cancer using array-based comparative genomic hybridization Naylor, Tara L.; Greshock, Joel; Wang, Yan Breast Cancer Research, Vol. 7, Issue 6 https://doi.org/10.1186/bcr1356	journal	November 2005
Immunohistochemical study of nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 in invasive breast carcinoma of no special type Symonowicz, Krzysztof; DuŚ-Szachniewicz, Kamila; WoŹNiak, Marta Experimental and Therapeutic Medicine, Vol. 8, Issue 4 https://doi.org/10.3892/etm.2014.1847	journal	July 2014
KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers Corson, Timothy W.; Huang, Annie; Tsao, Ming-Sound Oncogene, Vol. 24, Issue 30 https://doi.org/10.1038/sj.onc.1208641	journal	May 2005
NUCKS1 overexpression is a novel biomarker for recurrence-free survival in cervical squamous cell carcinoma Gu, Lina; Xia, Bairong; Zhong, Lili Tumor Biology, Vol. 35, Issue 8 https://doi.org/10.1007/s13277-014-2035-5	journal	May 2014
Cooperation of breast cancer proteins PALB2 and piccolo BRCA2 in stimulating homologous recombination Buisson, Rémi; Dion-Côté, Anne-Marie; Coulombe, Yan Nature Structural & Molecular Biology, Vol. 17, Issue 10 https://doi.org/10.1038/nsmb.1915	journal	September 2010
Breast Cancer Proteins PALB2 and BRCA2 Stimulate Polymerase η in Recombination-Associated DNA Synthesis at Blocked Replication Forks Buisson, Rémi; Niraj, Joshi; Pauty, Joris Cell Reports, Vol. 6, Issue 3 https://doi.org/10.1016/j.celrep.2014.01.009	journal	February 2014
More forks on the road to replication stress recovery Allen, C.; Ashley, A. K.; Hromas, R. Journal of Molecular Cell Biology, Vol. 3, Issue 1 https://doi.org/10.1093/jmcb/mjq049	journal	January 2011
Pathways of mammalian replication fork restart Petermann, Eva; Helleday, Thomas Nature Reviews Molecular Cell Biology, Vol. 11, Issue 10 https://doi.org/10.1038/nrm2974	journal	September 2010
Dormant origins licensed by excess Mcm2 7 are required for human cells to survive replicative stress Ge, X. Q.; Jackson, D. A.; Blow, J. J. Genes & Development, Vol. 21, Issue 24 https://doi.org/10.1101/gad.457807	journal	December 2007
Dynamics of DNA Replication in Mammalian Somatic Cells Anglana, Mauro; Apiou, Françoise; Bensimon, Aaron Cell, Vol. 114, Issue 3 https://doi.org/10.1016/S0092-8674(03)00569-5	journal	August 2003
A homologous recombination defect affects replication-fork progression in mammalian cells Daboussi, F.; Courbet, S.; Benhamou, S. Journal of Cell Science, Vol. 121, Issue 2 https://doi.org/10.1242/jcs.010330	journal	January 2008
DNA damage by X-rays and their impact on replication processes Parplys, Ann Christin; Petermann, Eva; Petersen, Cordula Radiotherapy and Oncology, Vol. 102, Issue 3 https://doi.org/10.1016/j.radonc.2012.01.005	journal	March 2012
Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells Zellweger, Ralph; Dalcher, Damian; Mutreja, Karun Journal of Cell Biology, Vol. 208, Issue 5 https://doi.org/10.1083/jcb.201406099	journal	March 2015
Topoisomerase I poisoning results in PARP-mediated replication fork reversal Ray Chaudhuri, Arnab; Hashimoto, Yoshitami; Herrador, Raquel Nature Structural & Molecular Biology, Vol. 19, Issue 4 https://doi.org/10.1038/nsmb.2258	journal	March 2012
Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery Murphy, Anar K.; Fitzgerald, Michael; Ro, Teresa Journal of Cell Biology, Vol. 206, Issue 4 https://doi.org/10.1083/jcb.201404111	journal	August 2014
Human RPA phosphorylation by ATR stimulates DNA synthesis and prevents ssDNA accumulation during DNA-replication stress Vassin, V. M.; Anantha, R. W.; Sokolova, E. Journal of Cell Science, Vol. 122, Issue 22 https://doi.org/10.1242/jcs.053702	journal	October 2009
DNA-PK-Dependent RPA2 Hyperphosphorylation Facilitates DNA Repair and Suppresses Sister Chromatid Exchange Liaw, Hungjiun; Lee, Deokjae; Myung, Kyungjae PLoS ONE, Vol. 6, Issue 6 https://doi.org/10.1371/journal.pone.0021424	journal	June 2011
A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response Adamson, Britt; Smogorzewska, Agata; Sigoillot, Frederic D. Nature Cell Biology, Vol. 14, Issue 3 https://doi.org/10.1038/ncb2426	journal	February 2012
BRCA1 regulation of transcription Rosen, Eliot M.; Fan, Saijun; Ma, Yongxian Cancer Letters, Vol. 236, Issue 2 https://doi.org/10.1016/j.canlet.2005.04.037	journal	May 2006
Genome-wide analysis reveals a role for BRCA1 and PALB2 in transcriptional co-activation Gardini, A.; Baillat, D.; Cesaroni, M. The EMBO Journal, Vol. 33, Issue 8 https://doi.org/10.1002/embj.201385567	journal	March 2014
A putative DNA-binding domain in the NUCKS protein Grundt, Kirsten; Skjeldal, Lars; Anthonsen, Henrik W. Archives of Biochemistry and Biophysics, Vol. 407, Issue 2 https://doi.org/10.1016/s0003-9861(02)00513-1	journal	November 2002
Expression of Human Telomerase (hTERT) Does Not Prevent Stress-induced Senescence in Normal Human Fibroblasts but Protects the Cells from Stress-induced Apoptosis and Necrosis Gorbunova, Vera; Seluanov, Andrei; Pereira-Smith, Olivia M. Journal of Biological Chemistry, Vol. 277, Issue 41 https://doi.org/10.1074/jbc.m202671200	journal	July 2002
Immunohistochemical and Proteomic Evaluation of Nuclear Ubiquitous Casein and Cyclin-Dependent Kinases Substrate in Invasive Ductal Carcinoma of the Breast Ziółkowski, Piotr; Gamian, Elżbieta; Osiecka, Beata Journal of Biomedicine and Biotechnology, Vol. 2009 https://doi.org/10.1155/2009/919645	journal	January 2009
Discovery of Novel Methylation Biomarkers in Cervical Carcinoma by Global Demethylation and Microarray Analysis Sova, P. Cancer Epidemiology Biomarkers & Prevention, Vol. 15, Issue 1 https://doi.org/10.1158/1055-9965.epi-05-0323	journal	January 2006

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59 BASIC BIOLOGICAL SCIENCES

Title: NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability

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