Crystal structure and interaction studies of the human FBxo3 ApaG domain

Krzysiak, Troy C.; Chen, Bill B.; Lear, Travis; Mallampalli, Rama K.; Gronenborn, Angela M.

doi:10.1111/febs.13721

Title: Crystal structure and interaction studies of the human FBxo3 ApaG domain

Journal Article · Fri Apr 22 00:00:00 EDT 2016 · Federation of European Biochemical Societies (FEBS) Journal

DOI:https://doi.org/10.1111/febs.13721· OSTI ID:1256364

Krzysiak, Troy C. ^[1]; Chen, Bill B. ^[1]; Lear, Travis ^[1]; Mallampalli, Rama K. ^[1]; Gronenborn, Angela M. ^[1]

Univ. of Pittsburgh, PA (United States)

Transcriptional activation of proinflammatory cytokines, mediated by tumor necrosis factor receptor-associated factors (TRAFs), is in part triggered by the degradation of the F-box protein, FBxl2, via an E3 ligase that contains another F-box protein, FBxo3. The ApaG domain of FBxo3 is required for the interaction with and degradation of FBxl2 [Mallampalli RK et al., (2013) J Immunol 191, 5247–5255]. Here, we report the X-ray structure of the human FBxo3 ApaG domain, residues 278–407, at 2.0 Å resolution. Like bacterial ApaG proteins, this domain is characterized by a classic Immunoglobin/Fibronectin III-type fold, comprising a seven-stranded β-sheet core, surrounded by four extended loops. Although cation binding had been proposed for bacterial ApaG proteins, no interactions with Mg²⁺ or Co²⁺ were detected for the human ApaG domain. In addition, dinucleotide polyphosphates, which have been reported to be second messengers in the inflammation response and targets of the bacterial apaG-containing operon, are not bound by the human ApaG domain. In the context of the full-length protein, loop 1, comprising residues 294-303, is critical for the interaction with FBxl2. However, titration of the individual ApaG domain with a 15-mer FBxl2 peptide that was phosphorylated on the crucial T404, as well as the inability of the ApaG domain to interact with full-length FBxl2, assessed by coimmunoprecipitation, indicate that the ApaG domain alone is necessary, but not sufficient for binding and degradation of FBxl2.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: HL116472; HL096376; HL097376; HL098174; HL081784; HL114453

OSTI ID:: 1256364

Journal Information:: Federation of European Biochemical Societies (FEBS) Journal, Vol. 283, Issue 11; ISSN 1742-464X

Publisher:: Federation of European Biochemical SocietiesCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 6 works

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59 BASIC BIOLOGICAL SCIENCES
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inflammation

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