Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry

Ophus, Colin; Ciston, Jim; Pierce, Jordan; Harvey, Tyler R.; Chess, Jordan; McMorran, Benjamin J.; Czarnik, Cory; Rose, Harald H.; Ercius, Peter

doi:10.1038/ncomms10719

Title: Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry

Journal Article · Mon Feb 29 00:00:00 EST 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms10719· OSTI ID:1255550

Ophus, Colin ^[1]; Ciston, Jim ^[1]; Pierce, Jordan ^[2]; Harvey, Tyler R. ^[2]; Chess, Jordan ^[2]; McMorran, Benjamin J. ^[2]; Czarnik, Cory ^[3]; Rose, Harald H. ^[4]; Ercius, Peter ^[1]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of Oregon, Eugene, OR (United States)
Gatan Inc., Pleasanton, CA (United States)
Ulm Univ., Ulm (Germany)

The ability to image light elements in soft matter at atomic resolution enables unprecedented insight into the structure and properties of molecular heterostructures and beam-sensitive nanomaterials. In this study, we introduce a scanning transmission electron microscopy technique combining a pre-specimen phase plate designed to produce a probe with structured phase with a high-speed direct electron detector to generate nearly linear contrast images with high efficiency. We demonstrate this method by using both experiment and simulation to simultaneously image the atomic-scale structure of weakly scattering amorphous carbon and strongly scattering gold nanoparticles. Our method demonstrates strong contrast for both materials, making it a promising candidate for structural determination of het erogeneous soft/hard matter samples even at low electron doses comparable to traditional phase-contrast transmission electron microscopy. Simulated images demonstrate the extension of this technique to the challenging problem of structural determination of biological material at the surface of inorganic crystals.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231; SC0010466

OSTI ID:: 1255550

Alternate ID(s):: OSTI ID: 1379111

Journal Information:: Nature Communications, Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 66 works

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