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Title: Contributions of Immunoaffinity Chromatography to Deep Proteome Profiling of Human Biofluids

Human biofluids, especially blood plasma or serum, hold great potential as the sources of potential biomarkers for various diseases; however, the enormous dynamic range of protein concentrations in biofluids represents a significant analytical challenge to detect promising low-abundance protein biomarkers. Over the last decade, various immunoaffinity chromatographic methods have been developed and routinely applied for separating low-abundance proteins from the high and moderate-abundance proteins, thus enabling more effective detection of low-abundance proteins. Herein, we review the advances of immunoaffinity separation methods and their contributions to the proteomics applications of different human biofluids. The limitations and future perspective of immunoaffinity separation methods are also discussed.
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Publication Date:
OSTI Identifier:
Report Number(s):
48135; 46197; 40991; 48666; 453040220
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Chromatography B, 1021:57-68
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
Country of Publication:
United States
Environmental Molecular Sciences Laboratory