Proteomic analysis reveals metabolic and regulatory systems involved in the syntrophic and axenic lifestyle of Syntrophomonas wolfei

Sieber, Jessica R.; Crable, Bryan R.; Sheik, Cody S.; Hurst, Gregory B.; Rohlin, Lars; Gunsalus, Robert P.; McInerney, Michael J.

doi:10.3389/fmicb.2015.00115

Title: Proteomic analysis reveals metabolic and regulatory systems involved in the syntrophic and axenic lifestyle of Syntrophomonas wolfei

Journal Article · Wed Feb 11 00:00:00 EST 2015 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2015.00115· OSTI ID:1254004

Sieber, Jessica R. ^[1]; Crable, Bryan R. ^[1]; Sheik, Cody S. ^[2]; Hurst, Gregory B. ^[3]; Rohlin, Lars ^[4]; Gunsalus, Robert P. ^[4]; McInerney, Michael J. ^[1]

Univ. of Oklahoma, Norman, OK (United States). Dept. of Botany and Microbiology
Univ. of Michigan, Ann Arbor, MI (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
Univ. of California, Los Angeles, CA (United States)

Microbial syntrophy is a vital metabolic interaction necessary for the complete oxidation of organic biomass to methane in all-anaerobic ecosystems. However, this process is thermodynamically constrained and represents an ecosystem-level metabolic bottleneck. To gain insight into the physiology of this process, a shotgun proteomics approach was used to quantify the protein landscape of the model syntrophic metabolizer, Syntrophomonas wolfei, grown axenically and syntrophically with Methanospirillum hungatei. Remarkably, the abundance of most proteins as represented by normalized spectral abundance factor (NSAF) value changed very little between the pure and coculture growth conditions. Among the most abundant proteins detected were GroEL and GroES chaperonins, a small heat shock protein, and proteins involved in electron transfer, beta-oxidation, and ATP synthesis. Several putative energy conservation enzyme systems that utilize NADH and ferredoxin were present. The abundance of an EtfAB2 and the membrane-bound iron-sulfur oxidoreductase (Swol_0698 gene product) delineated a potential conduit for electron transfer between acyl-CoA dehydrogenases and membrane redox carriers. Proteins detected only when S. wolfei was grown with M. hungatei included a zinc-dependent dehydrogenase with a GroES domain, whose gene is present in genomes in many organisms capable of syntrophy, and transcriptional regulators responsive to environmental stimuli or the physiological status of the cell. The proteomic analysis revealed an emphasis on macromolecular stability and energy metabolism by S. wolfei and presence of regulatory mechanisms responsive to external stimuli and cellular physiological status.

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Research Organization:: Univ. of California, Los Angeles, CA (United States); Univ. of Oklahoma, Norman, OK (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: Univ. of Oklahoma, Norman, OK (United States); Univ. of California, Los Angeles, CA (United States)

Grant/Contract Number:: FC02-02ER63421; FG03-86ER13498; FG02-96ER20214; AC05-00OR22725

OSTI ID:: 1254004

Alternate ID(s):: OSTI ID: 1286726

Journal Information:: Frontiers in Microbiology, Vol. 6; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 37 works

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Related Subjects

60 APPLIED LIFE SCIENCES
59 BASIC BIOLOGICAL SCIENCES
syntrophy
Syntrophomonas wolfei
interspecies electron transfer
reverse electron transfer
hydrogen
methanogenesis