Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme

Journal Article · Thu Apr 07 00:00:00 EDT 2016 · Scientific Reports

DOI:https://doi.org/10.1038/srep24239· OSTI ID:1253915

Sherkhanov, Saken ^[1]; Korman, Tyler P. ^[1]; Clarke, Steven G. ^[1]; Bowie, James U. ^[1]

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1. Univ. of California, Los Angeles, CA (United States)

Here, most biodiesel currently in use consists of fatty acid methyl esters (FAMEs) produced by transesterification of plant oils with methanol. To reduce competition with food supplies, it would be desirable to directly produce biodiesel in microorganisms. To date, the most effective pathway for the production of biodiesel in bacteria yields fatty acid ethyl esters (FAEEs) at up to ~1.5 g/L. A much simpler route to biodiesel produces FAMEs by direct S-adenosyl-L-methionine (SAM) dependent methylation of free fatty acids, but FAME production by this route has been limited to only ~16 mg/L. Here we employ an alternative, broad spectrum methyltransferase, Drosophila melanogaster Juvenile Hormone Acid O-Methyltransferase (DmJHAMT). By introducing DmJHAMT in E. coli engineered to produce medium chain fatty acids and overproduce SAM, we obtain medium chain FAMEs at titers of 0.56 g/L, a 35-fold increase over titers previously achieved. Although considerable improvements will be needed for viable bacterial production of FAMEs and FAEEs for biofuels, it may be easier to optimize and transport the FAME production pathway to other microorganisms because it involves fewer enzymes.

• Research Organization: Univ. of California, Los Angeles, CA (United States)
• Sponsoring Organization: USDOE
• Grant/Contract Number: FC02-02ER63421
• OSTI ID: 1253915
• Journal Information: Scientific Reports, Vol. 6; ISSN 2045-2322
• Publisher: Nature Publishing Group
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
biocatalysis
enzymes
metabolic engineering