Structure-Based Design of Functional Amyloid Materials

Li, Dan; Jones, Eric M.; Sawaya, Michael R.; Furukawa, Hiroyasu; Luo, Fang; Ivanova, Magdalena; Sievers, Stuart A.; Wang, Wenyuan; Yaghi, Omar M.; Liu, Cong; Eisenberg, David S.

doi:10.1021/ja509648u

Title: Structure-Based Design of Functional Amyloid Materials

Journal Article · Thu Dec 04 00:00:00 EST 2014 · Journal of the American Chemical Society

DOI:https://doi.org/10.1021/ja509648u· OSTI ID:1252903

Li, Dan ^[1]; Jones, Eric M. ^[1]; Sawaya, Michael R. ^[1]; Furukawa, Hiroyasu ^[2]; Luo, Fang ^[3]; Ivanova, Magdalena ^[1]; Sievers, Stuart A. ^[1]; Wang, Wenyuan ^[3]; Yaghi, Omar M. ^[2]; Liu, Cong ^[3]; Eisenberg, David S. ^[1]

Univ. of California, Los Angeles, CA (United States)
Univ. of California, Los Angeles, CA (United States); Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Chinese Academy of Sciences (CAS), Shanghai (China). Shanghai Inst. of Organic Chemistry

We report that amyloid fibers, once exclusively associated with disease, are acquiring utility as a class of biological nanomaterials. We introduce a method that utilizes the atomic structures of amyloid peptides, to design materials with versatile applications. As a model application, we designed amyloid fibers capable of capturing carbon dioxide from flue gas, to address the global problem of excess anthropogenic carbon dioxide. By measuring dynamic separation of carbon dioxide from nitrogen, we show that fibers with designed amino acid sequences double the carbon dioxide binding capacity of the previously reported fiber formed by VQIVYK from Tau protein. In a second application, we designed fibers that facilitate retroviral gene transfer. Finally, by measuring lentiviral transduction, we show that designed fibers exceed the efficiency of polybrene, a commonly used enhancer of transduction. The same procedures can be adapted to the design of countless other amyloid materials with a variety of properties and uses.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FCO-02ER-63421; MCB 0958111

OSTI ID:: 1252903

Journal Information:: Journal of the American Chemical Society, Vol. 136, Issue 52; ISSN 0002-7863

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 84 works

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