Antibodies to a conformational epitope on gp41 neutralize HIV-1 by destabilizing the Env spike

Lee, Jeong Hyun; Leaman, Daniel P.; Kim, Arthur S.; Torrents de la Peña, Alba; Sliepen, Kwinten; Yasmeen, Anila; Derking, Ronald; Ramos, Alejandra; de Taeye, Steven W.; Ozorowski, Gabriel; Klein, Florian; Burton, Dennis R.; Nussenzweig, Michel C.; Poignard, Pascal; Moore, John P.; Klasse, Per Johan; Sanders, Rogier W.; Zwick, Michael B.; Wilson, Ian A.; Ward, Andrew B.

doi:10.1038/ncomms9167

Title: Antibodies to a conformational epitope on gp41 neutralize HIV-1 by destabilizing the Env spike

Journal Article · Fri Sep 25 00:00:00 EDT 2015 · Nature Communications

DOI:https://doi.org/10.1038/ncomms9167· OSTI ID:1252760

Lee, Jeong Hyun ^[1]; Leaman, Daniel P. ^[1]; Kim, Arthur S. ^[1]; Torrents de la Peña, Alba ^[2]; Sliepen, Kwinten ^[2]; Yasmeen, Anila ^[3]; Derking, Ronald ^[2]; Ramos, Alejandra ^[1]; de Taeye, Steven W. ^[2]; Ozorowski, Gabriel ^[1]; Klein, Florian ^[4]; Burton, Dennis R. ^[5]; Nussenzweig, Michel C. ^[4]; Poignard, Pascal ^[1]; Moore, John P. ^[3]; Klasse, Per Johan ^[3]; Sanders, Rogier W. ^[6]; Zwick, Michael B. ^[1]; Wilson, Ian A. ^[1]; Ward, Andrew B. ^[1]

The Scripps Research Inst., La Jolla, CA (United States)
Academic Medical Center, Amsterdam, AZ (The Netherlands)
Weill Medical College of Cornell Univ., New York, NY (United States)
The Rockefeller Univ., New York, NY (United States)
The Scripps Research Inst., La Jolla, CA (United States); Ragon Institute of MGH, MIT and Harvard, Boston, MA (United States)
Academic Medical Center, Amsterdam, AZ (The Netherlands); Weill Medical College of Cornell University, New York, NY (United States)

The recent identification of three broadly neutralizing antibodies (bnAbs) against gp120–gp41 interface epitopes has expanded the targetable surface on the HIV-1 envelope glycoprotein (Env) trimer. By using biochemical, biophysical and computational methods, we map the previously unknown trimer epitopes of two related antibodies, 3BC315 and 3BC176. A cryo-EM reconstruction of a soluble Env trimer bound to 3BC315 Fab at 9.3 Å resolution reveals that the antibody binds between two gp41 protomers, and neutralizes the virus by accelerating trimer decay. In contrast, bnAb 35O22 binding to a partially overlapping quaternary epitope at the gp120–gp41 interface does not induce decay. A conserved gp41-proximal glycan at N88 was also shown to play a role in the binding kinetics of 3BC176 and 3BC315. Finally, our data suggest that the dynamic structure of the Env trimer influences exposure of bnAb epitopes.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); U.S. National Inst. of Health (NIH); National Inst. of General Medical Sciences (NIGMS); Howard Hughes Medical Inst.

Grant/Contract Number:: AC02-05CH11231; U54 GM094586; RR017573; P01 AI082362; UM1 AI100663; R01 AI084817; R01 AI114401; R01 AI098602

OSTI ID:: 1252760

Journal Information:: Nature Communications, Vol. 6, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

Citation Metrics:

Cited by: 61 works

Citation information provided by
Web of Science

References (65)

Structure and immune recognition of trimeric pre-fusion HIV-1 Env Pancera, Marie; Zhou, Tongqing; Druz, Aliaksandr Nature, Vol. 514, Issue 7523 https://doi.org/10.1038/nature13808	journal	October 2014
Structural Families in Loops of Homologous Proteins: Automatic Classification, Modelling and Application to Antibodies Martin, Andrew C. R.; Thornton, Janet M. Journal of Molecular Biology, Vol. 263, Issue 5 https://doi.org/10.1006/jmbi.1996.0617	journal	November 1996
Atomic structure of the ectodomain from HIV-1 gp41 Weissenhorn, W.; Dessen, A.; Harrison, S. C. Nature, Vol. 387, Issue 6631 https://doi.org/10.1038/387426a0	journal	May 1997
Broad and potent neutralization of HIV-1 by a gp41-specific human antibody Huang, Jinghe; Ofek, Gilad; Laub, Leo Nature, Vol. 491, Issue 7424 https://doi.org/10.1038/nature11544	journal	September 2012
Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9 McLellan, Jason S.; Pancera, Marie; Carrico, Chris Nature, Vol. 480, Issue 7377 https://doi.org/10.1038/nature10696	journal	November 2011
Broad and potent HIV-1 neutralization by a human antibody that binds the gp41–gp120 interface Huang, Jinghe; Kang, Byong H.; Pancera, Marie Nature, Vol. 515, Issue 7525 https://doi.org/10.1038/nature13601	journal	September 2014
Broadly Neutralizing Antibody PGT121 Allosterically Modulates CD4 Binding via Recognition of the HIV-1 gp120 V3 Base and Multiple Surrounding Glycans Julien, Jean-Philippe; Sok, Devin; Khayat, Reza PLoS Pathogens, Vol. 9, Issue 5 https://doi.org/10.1371/journal.ppat.1003342	journal	May 2013
Antibody 8ANC195 Reveals a Site of Broad Vulnerability on the HIV-1 Envelope Spike Scharf, Louise; Scheid, Johannes F.; Lee, Jeong Hyun Cell Reports, Vol. 7, Issue 3 https://doi.org/10.1016/j.celrep.2014.04.001	journal	May 2014
Broad neutralization coverage of HIV by multiple highly potent antibodies Walker, Laura M.; Huber, Michael; Doores, Katie J. Nature, Vol. 477, Issue 7365 https://doi.org/10.1038/nature10373	journal	September 2011
Accurate determination of local defocus and specimen tilt in electron microscopy Mindell, Joseph A.; Grigorieff, Nikolaus Journal of Structural Biology, Vol. 142, Issue 3 https://doi.org/10.1016/S1047-8477(03)00069-8	journal	June 2003
DoG Picker and TiltPicker: Software tools to facilitate particle selection in single particle electron microscopy Voss, N. R.; Yoshioka, C. K.; Radermacher, M. Journal of Structural Biology, Vol. 166, Issue 2 https://doi.org/10.1016/j.jsb.2009.01.004	journal	May 2009
Structural Delineation of a Quaternary, Cleavage-Dependent Epitope at the gp41-gp120 Interface on Intact HIV-1 Env Trimers Blattner, Claudia; Lee, Jeong Hyun; Sliepen, Kwinten Immunity, Vol. 40, Issue 5 https://doi.org/10.1016/j.immuni.2014.04.008	journal	May 2014
Prevention of overfitting in cryo-EM structure determination Scheres, Sjors H. W.; Chen, Shaoxia Nature Methods, Vol. 9, Issue 9 https://doi.org/10.1038/nmeth.2115	journal	July 2012
Broad neutralization by a combination of antibodies recognizing the CD4 binding site and a new conformational epitope on the HIV-1 envelope protein Klein, Florian; Gaebler, Christian; Mouquet, Hugo The Journal of Experimental Medicine, Vol. 209, Issue 8 https://doi.org/10.1084/jem.20120423	journal	July 2012
Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding Scheid, J. F.; Mouquet, H.; Ueberheide, B. Science, Vol. 333, Issue 6049 https://doi.org/10.1126/science.1207227	journal	July 2011
Conformations of immunoglobulin hypervariable regions Chothia, Cyrus; Lesk, Arthur M.; Tramontano, Anna Nature, Vol. 342, Issue 6252 https://doi.org/10.1038/342877a0	journal	December 1989
Structure-Based Design of a Fusion Glycoprotein Vaccine for Respiratory Syncytial Virus McLellan, J. S.; Chen, M.; Joyce, M. G. Science, Vol. 342, Issue 6158 https://doi.org/10.1126/science.1243283	journal	October 2013
Inhibition of the HIV-1 Spike by Single-PG9/16-Antibody Binding Suggests a Coordinated-Activation Model for Its Three Protomeric Units Loving, R.; Sjoberg, M.; Wu, S. -R. Journal of Virology, Vol. 87, Issue 12 https://doi.org/10.1128/JVI.00530-13	journal	April 2013
Broadly Neutralizing HIV Antibodies Define a Glycan-Dependent Epitope on the Prefusion Conformation of gp41 on Cleaved Envelope Trimers Falkowska, Emilia; Le, Khoa M.; Ramos, Alejandra Immunity, Vol. 40, Issue 5 https://doi.org/10.1016/j.immuni.2014.04.009	journal	May 2014
A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies Sanders, Rogier W.; Derking, Ronald; Cupo, Albert PLoS Pathogens, Vol. 9, Issue 9, Article No. e1003618 https://doi.org/10.1371/journal.ppat.1003618	journal	September 2013
Viral Escape from HIV-1 Neutralizing Antibodies Drives Increased Plasma Neutralization Breadth through Sequential Recognition of Multiple Epitopes and Immunotypes Wibmer, Constantinos Kurt; Bhiman, Jinal N.; Gray, Elin S. PLoS Pathogens, Vol. 9, Issue 10 https://doi.org/10.1371/journal.ppat.1003738	journal	October 2013
Crystal Structure of a Soluble Cleaved HIV-1 Envelope Trimer Julien, J. -P.; Cupo, A.; Sok, D. Science, Vol. 342, Issue 6165 https://doi.org/10.1126/science.1245625	journal	October 2013
Rational HIV Immunogen Design to Target Specific Germline B Cell Receptors Jardine, J.; Julien, J. -P.; Menis, S. Science, Vol. 340, Issue 6133 https://doi.org/10.1126/science.1234150	journal	March 2013
Cryo-EM Structure of a Fully Glycosylated Soluble Cleaved HIV-1 Envelope Trimer Lyumkis, D.; Julien, J. -P.; de Val, N. Science, Vol. 342, Issue 6165 https://doi.org/10.1126/science.1245627	journal	October 2013
A Blueprint for HIV Vaccine Discovery Burton, Dennis R.; Ahmed, Rafi; Barouch, Dan H. Cell Host & Microbe, Vol. 12, Issue 4 https://doi.org/10.1016/j.chom.2012.09.008	journal	October 2012
Cleavage strongly influences whether soluble HIV-1 envelope glycoprotein trimers adopt a native-like conformation Ringe, R. P.; Sanders, R. W.; Yasmeen, A. Proceedings of the National Academy of Sciences, Vol. 110, Issue 45 https://doi.org/10.1073/pnas.1314351110	journal	October 2013
Recombinant HIV envelope trimer selects for quaternary-dependent antibodies targeting the trimer apex Sok, Devin; van Gils, Marit J.; Pauthner, Matthias Proceedings of the National Academy of Sciences, Vol. 111, Issue 49 https://doi.org/10.1073/pnas.1415789111	journal	November 2014
Rational Design of Envelope Identifies Broadly Neutralizing Human Monoclonal Antibodies to HIV-1 Wu, X.; Yang, Z. -Y.; Li, Y. Science, Vol. 329, Issue 5993 https://doi.org/10.1126/science.1187659	journal	July 2010
Promiscuous Glycan Site Recognition by Antibodies to the High-Mannose Patch of gp120 Broadens Neutralization of HIV Sok, D.; Doores, K. J.; Briney, B. Science Translational Medicine, Vol. 6, Issue 236 https://doi.org/10.1126/scitranslmed.3008104	journal	May 2014
MPER-specific antibodies induce gp120 shedding and irreversibly neutralize HIV-1 Ruprecht, Claudia R.; Krarup, Anders; Reynell, Lucy The Journal of Experimental Medicine, Vol. 208, Issue 3 https://doi.org/10.1084/jem.20101907	journal	February 2011
Antibody to gp41 MPER Alters Functional Properties of HIV-1 Env without Complete Neutralization Kim, Arthur S.; Leaman, Daniel P.; Zwick, Michael B. PLoS Pathogens, Vol. 10, Issue 7 https://doi.org/10.1371/journal.ppat.1004271	journal	July 2014
Asymmetric recognition of the HIV-1 trimer by broadly neutralizing antibody PG9 Julien, Jean-Philippe; Lee, Jeong Hyun; Cupo, Albert Proceedings of the National Academy of Sciences, Vol. 110, Issue 11 https://doi.org/10.1073/pnas.1217537110	journal	February 2013
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Partial Enzymatic Deglycosylation Preserves the Structure of Cleaved Recombinant HIV-1 Envelope Glycoprotein Trimers Depetris, Rafael S.; Julien, Jean-Philippe; Khayat, Reza Journal of Biological Chemistry, Vol. 287, Issue 29 https://doi.org/10.1074/jbc.M112.371898	journal	May 2012
Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01 Zhou, T.; Georgiev, I.; Wu, X. Science, Vol. 329, Issue 5993 https://doi.org/10.1126/science.1192819	journal	July 2010
A clustering approach to multireference alignment of single-particle projections in electron microscopy Sorzano, C. O. S.; Bilbao-Castro, J. R.; Shkolnisky, Y. Journal of Structural Biology, Vol. 171, Issue 2 https://doi.org/10.1016/j.jsb.2010.03.011	journal	August 2010
Conformational dynamics of single HIV-1 envelope trimers on the surface of native virions Munro, J. B.; Gorman, J.; Ma, X. Science, Vol. 346, Issue 6210 https://doi.org/10.1126/science.1254426	journal	October 2014
Cooperation of B Cell Lineages in Induction of HIV-1-Broadly Neutralizing Antibodies Gao, Feng; Bonsignori, Mattia; Liao, Hua-Xin Cell, Vol. 158, Issue 3 https://doi.org/10.1016/j.cell.2014.06.022	journal	July 2014
A Bayesian View on Cryo-EM Structure Determination Scheres, Sjors H. W. Journal of Molecular Biology, Vol. 415, Issue 2 https://doi.org/10.1016/j.jmb.2011.11.010	journal	January 2012
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Broad and Potent Neutralizing Antibodies from an African Donor Reveal a New HIV-1 Vaccine Target Walker, L. M.; Phogat, S. K.; Chan-Hui, P. -Y. Science, Vol. 326, Issue 5950 https://doi.org/10.1126/science.1178746	journal	September 2009
CD4-Induced Activation in a Soluble HIV-1 Env Trimer Guttman, Miklos; Garcia, Natalie K.; Cupo, Albert Structure, Vol. 22, Issue 7 https://doi.org/10.1016/j.str.2014.05.001	journal	July 2014
Accelerating Next-Generation Vaccine Development for Global Disease Prevention Koff, W. C.; Burton, D. R.; Johnson, P. R. Science, Vol. 340, Issue 6136 https://doi.org/10.1126/science.1232910	journal	May 2013
RELION: Implementation of a Bayesian approach to cryo-EM structure determination Scheres, Sjors H. W. Journal of Structural Biology, Vol. 180, Issue 3, p. 519-530 https://doi.org/10.1016/j.jsb.2012.09.006	journal	December 2012
Engineering HIV envelope protein to activate germline B cell receptors of broadly neutralizing anti-CD4 binding site antibodies McGuire, Andrew T.; Hoot, Sam; Dreyer, Anita M. The Journal of Experimental Medicine, Vol. 210, Issue 4 https://doi.org/10.1084/jem.20122824	journal	March 2013
A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype B env Gene Pugach, Pavel; Ozorowski, Gabriel; Cupo, Albert Journal of Virology, Vol. 89, Issue 6 https://doi.org/10.1128/JVI.03473-14	journal	January 2015
Differential binding of neutralizing and non-neutralizing antibodies to native-like soluble HIV-1 Env trimers, uncleaved Env proteins, and monomeric subunits Yasmeen, Anila; Ringe, Rajesh; Derking, Ronald Retrovirology, Vol. 11, Issue 1 https://doi.org/10.1186/1742-4690-11-41	journal	January 2014
HIV-1 Envelope Glycoprotein Trimers Display Open Quaternary Conformation When Bound to the gp41 Membrane-Proximal External-Region-Directed Broadly Neutralizing Antibody Z13e1 Harris, A. K.; Bartesaghi, A.; Milne, J. L. S. Journal of Virology, Vol. 87, Issue 12 https://doi.org/10.1128/JVI.03284-12	journal	April 2013
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Refinement of Protein Structures into Low-Resolution Density Maps Using Rosetta DiMaio, Frank; Tyka, Michael D.; Baker, Matthew L. Journal of Molecular Biology, Vol. 392, Issue 1 https://doi.org/10.1016/j.jmb.2009.07.008	journal	September 2009
Structural Insights on the Role of Antibodies in HIV-1 Vaccine and Therapy West, Anthony P.; Scharf, Louise; Scheid, Johannes F. Cell, Vol. 156, Issue 4 https://doi.org/10.1016/j.cell.2014.01.052	journal	February 2014
Comprehensive Antigenic Map of a Cleaved Soluble HIV-1 Envelope Trimer Derking, Ronald; Ozorowski, Gabriel; Sliepen, Kwinten PLOS Pathogens, Vol. 11, Issue 3 https://doi.org/10.1371/journal.ppat.1004767	journal	March 2015
Structural Evolution of Glycan Recognition by a Family of Potent HIV Antibodies Garces, Fernando; Sok, Devin; Kong, Leopold Cell, Vol. 159, Issue 1 https://doi.org/10.1016/j.cell.2014.09.009	journal	September 2014
Somatic Mutations of the Immunoglobulin Framework Are Generally Required for Broad and Potent HIV-1 Neutralization Klein, Florian; Diskin, Ron; Scheid, Johannes F. Cell, Vol. 153, Issue 1 https://doi.org/10.1016/j.cell.2013.03.018	journal	March 2013
Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120 Kong, Leopold; Lee, Jeong Hyun; Doores, Katie J. Nature Structural & Molecular Biology, Vol. 20, Issue 7 https://doi.org/10.1038/nsmb.2594	journal	May 2013
Leginon: An Automated System for Acquisition of Images from Vitreous Ice Specimens Carragher, Bridget; Kisseberth, Nick; Kriegman, David Journal of Structural Biology, Vol. 132, Issue 1 https://doi.org/10.1006/jsbi.2000.4314	journal	October 2000
Expressed Murine and Human CDR-H3 Intervals of Equal Length Exhibit Distinct Repertoires that Differ in their Amino Acid Composition and Predicted Range of Structures Zemlin, Michael; Klinger, Martin; Link, Jason Journal of Molecular Biology, Vol. 334, Issue 4 https://doi.org/10.1016/j.jmb.2003.10.007	journal	December 2003
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
Structural repertoire of the human VH segments Chothia, Cyrus; Lesk, Arthur M.; Gherardi, Ermanno Journal of Molecular Biology, Vol. 227, Issue 3 https://doi.org/10.1016/0022-2836(92)90224-8	journal	October 1992
A Conformational Switch in Human Immunodeficiency Virus gp41 Revealed by the Structures of Overlapping Epitopes Recognized by Neutralizing Antibodies Pejchal, R.; Gach, J. S.; Brunel, F. M. Journal of Virology, Vol. 83, Issue 17 https://doi.org/10.1128/JVI.00685-09	journal	June 2009
B Cell Liaison Confounds HIV-1 Evolution McHeyzer-Williams, Michael G. Cell, Vol. 158, Issue 3 https://doi.org/10.1016/j.cell.2014.07.018	journal	July 2014
A stamp on the envelope Sanders, Rogier W.; Moore, John P. Nature, Vol. 514, Issue 7523 https://doi.org/10.1038/nature13926	journal	October 2014
A Bayesian view on cryo-EM structure determination Scheres, Sjors H. W. 2012 IEEE 9th International Symposium on Biomedical Imaging (ISBI 2012), 2012 9th IEEE International Symposium on Biomedical Imaging (ISBI) https://doi.org/10.1109/isbi.2012.6235807	conference	May 2012
Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Antibodies 2F5 and 4E10 Require Surprisingly Few Crucial Residues in the Membrane-Proximal External Region of Glycoprotein gp41 To Neutralize HIV-1 Zwick, M. B.; Jensen, R.; Church, S. Journal of Virology, Vol. 79, Issue 2 https://doi.org/10.1128/jvi.79.2.1252-1261.2005	journal	December 2004
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010

Cited By (21)

Transient opening of trimeric prefusion RSV F proteins Gilman, Morgan S. A.; Furmanova-Hollenstein, Polina; Pascual, Gabriel Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-09807-5	journal	May 2019
Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence Sliepen, Kwinten; Han, Byung Woo; Bontjer, Ilja Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-10262-5	journal	May 2019
Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle Brouwer, Philip J. M.; Antanasijevic, Aleksandar; Berndsen, Zachary Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-12080-1	journal	September 2019
Non-neutralizing Antibodies Targeting the V1V2 Domain of HIV Exhibit Strong Antibody-Dependent Cell-mediated Cytotoxic Activity Mayr, Luzia M.; Decoville, Thomas; Schmidt, Sylvie Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-12883-6	journal	October 2017
Strategies for inducing effective neutralizing antibody responses against HIV-1 del Moral-Sánchez, Iván; Sliepen, Kwinten Expert Review of Vaccines, Vol. 18, Issue 11 https://doi.org/10.1080/14760584.2019.1690458	journal	November 2019
Adaption of human antibody λ and κ light chain architectures to CDR repertoires van der Kant, Rob; Bauer, Joschka; Karow-Zwick, Anne R. Protein Engineering, Design and Selection, Vol. 32, Issue 3 https://doi.org/10.1093/protein/gzz012	journal	March 2019
Cryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer Lee, J. H.; Ozorowski, G.; Ward, A. B. Science, Vol. 351, Issue 6277 https://doi.org/10.1126/science.aad2450	journal	March 2016
Structural and molecular basis for Ebola virus neutralization by protective human antibodies Misasi, J.; Gilman, M. S. A.; Kanekiyo, M. Science, Vol. 351, Issue 6279 https://doi.org/10.1126/science.aad6117	journal	February 2016
Recent advances in retroviruses via cryo-electron microscopy Mak, Johnson; de Marco, Alex Retrovirology, Vol. 15, Issue 1 https://doi.org/10.1186/s12977-018-0405-6	journal	February 2018
Engineering and characterising a novel, highly potent bispecific antibody iMab-CAP256 that targets HIV-1 Moshoette, Tumelo; Ali, Stuart Alvaro; Papathanasopoulos, Maria Antonia Retrovirology, Vol. 16, Issue 1 https://doi.org/10.1186/s12977-019-0493-y	journal	November 2019
CD4 occupancy triggers sequential pre-fusion conformational states of the HIV-1 envelope trimer with relevance for broadly neutralizing antibody activity Ivan, Branislav; Sun, Zhaozhi; Subbaraman, Harini PLOS Biology, Vol. 17, Issue 1 https://doi.org/10.1371/journal.pbio.3000114	journal	January 2019
Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins Schiffner, Torben; Pallesen, Jesper; Russell, Rebecca A. PLOS Pathogens, Vol. 14, Issue 5 https://doi.org/10.1371/journal.ppat.1006986	journal	May 2018
Glycoengineering HIV-1 Env creates ‘supercharged’ and ‘hybrid’ glycans to increase neutralizing antibody potency, breadth and saturation Crooks, Ema T.; Grimley, Samantha L.; Cully, Michelle PLOS Pathogens, Vol. 14, Issue 5 https://doi.org/10.1371/journal.ppat.1007024	journal	May 2018
In silico Identification of High-Affinity Ligands of the Hiv-1 Gp120 Protein, Potential Peptidomimetics of Neutralizing Antibody N6 Andrianov, A. M.; Nikolaev, G. I.; Kornoushenko, Y. V. Mathematical Biology and Bioinformatics, Vol. 14, Issue 2 https://doi.org/10.17537/2019.14.430	journal	October 2019
Development of Protein- and Peptide-Based HIV Entry Inhibitors Targeting gp120 or gp41 Pu, Jing; Wang, Qian; Xu, Wei Viruses, Vol. 11, Issue 8, p. 705 https://doi.org/10.3390/v11080705	journal	August 2019
Dynamic Viral Glycoprotein Machines: Approaches for Probing Transient States That Drive Membrane Fusion Garcia, Natalie; Lee, Kelly Viruses, Vol. 8, Issue 1 https://doi.org/10.3390/v8010015	journal	January 2016
HIV envelope trimer-elicited autologous neutralizing antibodies bind a region overlapping the N332 glycan supersite Nogal, Bartek; McCoy, Laura E.; van Gils, Marit J. Science Advances, Vol. 6, Issue 23 https://doi.org/10.1126/sciadv.aba0512	journal	June 2020
CD4 occupancy triggers sequential pre-fusion conformational states of the HIV-1 envelope trimer with relevance for broadly neutralizing antibody activity Ivan, Branislav; Sun, Zhaozhi; Subbaraman, Harini Public Library of Science (PLoS) https://doi.org/10.5167/uzh-166639	text	January 2019
Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability Dubrovskaya, Viktoriya; Tran, Karen; Ozorowski, Gabriel Immunity, Vol. 51, Issue 5 https://doi.org/10.1016/j.immuni.2019.10.008	journal	November 2019
Identification and specificity of broadly neutralizing antibodies against HIV McCoy, Laura E.; Burton, Dennis R. Immunological Reviews, Vol. 275, Issue 1 https://doi.org/10.1111/imr.12484	journal	January 2017
Chemical Cross-Linking Stabilizes Native-Like HIV-1 Envelope Glycoprotein Trimer Antigens Schiffner, Torben; de Val, Natalia; Russell, Rebecca A. Journal of Virology, Vol. 90, Issue 2 https://doi.org/10.1128/jvi.01942-15	journal	January 2016

Similar Records

Receptor Activation of HIV-1 Env Leads to Asymmetric Exposure of the gp41 Trimer

Journal Article · Mon Dec 19 00:00:00 EST 2016 · PLoS Pathogens · OSTI ID:1252760

Khasnis, Mukta D.; Halkidis, Konstantine; Bhardwaj, Anshul; +2 more

Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability

Journal Article · Tue Nov 12 00:00:00 EST 2019 · Immunity · OSTI ID:1252760

Dubrovskaya, Viktoriya; Tran, Karen; Ozorowski, Gabriel; +25 more

Structure and Recognition of a Novel HIV-1 gp120-gp41 Interface Antibody that Caused MPER Exposure through Viral Escape

Journal Article · Wed Jan 11 00:00:00 EST 2017 · PLoS Pathogens · OSTI ID:1252760

Wibmer, Constantinos Kurt; Gorman, Jason; Ozorowski, Gabriel; +20 more

Related Subjects

60 APPLIED LIFE SCIENCES
Antibodies
Cryoelectron microscopy
Molecular biophysics
X-ray crystallography

Title: Antibodies to a conformational epitope on gp41 neutralize HIV-1 by destabilizing the Env spike

Citation Formats

References (65)

Cited By (21)

Similar Records

Related Subjects