Protein-directed self-assembly of a fullerene crystal

Kim, Kook-Han; Ko, Dong-Kyun; Kim, Yong-Tae; Kim, Nam Hyeong; Paul, Jaydeep; Zhang, Shao-Qing; Murray, Christopher B.; Acharya, Rudresh; DeGrado, William F.; Kim, Yong Ho; Grigoryan, Gevorg

doi:10.1038/ncomms11429

Title: Protein-directed self-assembly of a fullerene crystal

Journal Article · Tue Apr 26 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms11429· OSTI ID:1251209

Kim, Kook-Han ^[1]; Ko, Dong-Kyun ^[2]; Kim, Yong-Tae ^[1]; Kim, Nam Hyeong ^[1]; Paul, Jaydeep ^[3]; Zhang, Shao-Qing ^[4]; Murray, Christopher B. ^[5]; Acharya, Rudresh ^[3]; DeGrado, William F. ^[6]; Kim, Yong Ho ^[7]; Grigoryan, Gevorg ^[8]

Sungkyunkwan Univ., Suwon (Korea)
New Jersey Inst. of Technology, Newark, NJ (United States)
National Inst. of Science Education and Research, Odisha (India)
Univ. of California, San Francisco, CA (United States); Univ. of Pennsylvania, Philadelphia, PA (United States)
Univ. of Pennsylvania, Philadelphia, PA (United States)
Univ. of California, San Francisco, CA (United States)
Sungkyunkwan Univ., Suwon (Korea); Inst. for Basic Science (IBS), Suwon (Korea)
Dartmouth College, Hanover, NH (United States)

Learning to engineer self-assembly would enable the precise organization of molecules by design to create matter with tailored properties. Here we demonstrate that proteins can direct the self-assembly of buckminsterfullerene (C₆₀) into ordered superstructures. A previously engineered tetrameric helical bundle binds C₆₀ in solution, rendering it water soluble. Two tetramers associate with one C₆₀, promoting further organization revealed in a 1.67-Å crystal structure. Fullerene groups occupy periodic lattice sites, sandwiched between two Tyr residues from adjacent tetramers. Strikingly, the assembly exhibits high charge conductance, whereas both the protein-alone crystal and amorphous C₆₀ are electrically insulating. The affinity of C₆₀ for its crystal-binding site is estimated to be in the nanomolar range, with lattices of known protein crystals geometrically compatible with incorporating the motif. Taken together, these findings suggest a new means of organizing fullerene molecules into a rich variety of lattices to generate new properties by design.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Science Foundation (NSF); National Institutes of Health (NIH); National Research Foundation of Korea (NRF); National Inst. of Science Education and Research; New Jersey Inst. of Technology

Grant/Contract Number:: GM54616; CHE-1413295; DMR 1120901; NRF-2014R1A1A2055647; NRF-2015M3C1A3002152; IBS-R015-D1

OSTI ID:: 1251209

Journal Information:: Nature Communications, Vol. 7, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 46 works

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