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Title: Final Report DE-SC0006997; PI Sharp; Coupled Biological and Micro-XAS/XRF Analysis of In Situ Uranium Biogeochemical Processes

Project Overview: The impact of the original seed award was substantially increased by leveraging a postdoctoral fellowship (Marie Curie Postdoctoral Fellowship) and parallel funds from (A) synergistic project supported by NSF and (B) with DOE collaborators (PI’s Ranville and Williams) as well as no-cost extension that greatly increased the impact and publications associated with the project. In aligning with SBR priorities, the project’s focus was extended more broadly to explore coupled biogeochemical analysis of metal (im)mobilization processes beyond uranium with a foundation in integrating microbial ecology with geochemical analyses. This included investigations of arsenic and zinc during sulfate reducing conditions in addition to direct microbial reduction of metals. Complimentary work with NSF funding and collaborative DOE interactions further increased the project scope to investigate metal (im)mobilization coupled to biogeochemical perturbations in forest ecosystems with an emphasis on coupled carbon and metal biogeochemistry. In total, the project was highly impactful and resulted in 9 publications and directly supported salary/tuition for 3 graduate students at various stages of their academic careers as well as my promotion to Associate Professor. In going forward, findings provided inspiration for a two subsequent proposals with collaborators at Lawrence Berkeley Laboratory and others that are currently inmore » review (as of March 2016).« less
  1. Colorado School of Mines, Golden, CO (United States)
Publication Date:
OSTI Identifier:
Report Number(s):
Final Report DE--SC0006997
DOE Contract Number:
Resource Type:
Technical Report
Resource Relation:
Related Information: 1. Drennan DM, Almstrand R, Lee I, Landkamer L, Figueroa L, Sharp JO (2016). Organoheterotrophic bacterial abundance associates with zinc removal in lignocellulose-based sulfate-reducing systems. Environ Sci Technol. 50(1): 378-87.2. Mikkelson KM, Lozupone CA, Sharp JO. (2016) Altered edaphic parameters couple to shifts in terrestrial bacterial community structure associated with insect-induced tree mortality. Soil Biology Biogeochem. 95:19-26 3. Almstrand R, Pinto AJ Figueroa LA, Sharp JO (2016) Draft genome of a novel Desulfobacteraceae member from a sulfate-reducing bioreactor metagenome. Genome Announcements 4(1) e01563-154. Pinto AJ, Sharp JO, Yoder MJ, Almstrand R (2016) Draft genome sequences of two novel acidimicrobiaceae from an acid mine drainage biofilm metagenome. Genome Announcements 4(1) e01563-155. Almstrand R, Drennan D, Sharp JO. (2015) Polygold FISH for signal amplification of metallo-labeled microbial cells. J Basic Microbio. 54:1-56. Stucker VK, Silverman DR, Williams KH, Sharp JO, Ranville JF. (2014) Thioarsenic species associated with increased arsenic release during biostimulated subsurface sulfate reduction. Environ Sci Technol. 48(22): 13367-75 7. Mikkelson KM, Bearup LA, Navarre-Sitchler AK, McCray JE, Sharp JO. (2014) Changes in metal mobility associated with bark beetle-induced tree mortality. Environ Sci: Processes Impacts. 16(6): 1318-27. 8. Bearup L, Mikkelson K, Wiley J, Navarre-Sitchler A, Maxwell R, Sharp JO, McCray J (2014). Metal fate and partitioning in soils under bark beetle-killed trees. Sci Total Environ. 496, 348-3579. Sharp JO, Schofield EJ, Lezama J, Ulrich K, Veeramani H, Junier P, Roquier C, Suvorova EI, Webb S, Tebo B, Giammar DE, Bargar JR, Bernier-Latmani R (2011). Uranium speciation and stability after reductive immobilization in sediments. Geochim Cosmochim Acta. 75(21):6497- 6510.
Research Org:
Colorado School of Mines, Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States