Phosphoketolase pathway engineering for carbon-efficient biocatalysis

Henard, Calvin Andrew; Freed, Emily Frances; Guarnieri, Michael Thomas

doi:10.1016/j.copbio.2015.08.018

Title: Phosphoketolase pathway engineering for carbon-efficient biocatalysis

Journal Article · Tue Sep 08 00:00:00 EDT 2015 · Current Opinion in Biotechnology

DOI:https://doi.org/10.1016/j.copbio.2015.08.018· OSTI ID:1485594

Henard, Calvin Andrew ^[1]; Freed, Emily Frances ^[1]; Guarnieri, Michael Thomas ^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)

Recent advances in metabolic engineering have facilitated the development of microbial biocatalysts capable of producing an array of bio-products, ranging from fuels to drug molecules. These bio-products are commonly generated through an acetyl-CoA intermediate, which serves as a key precursor in the biological conversion of carbon substrates. Conventional biocatalytic upgrading strategies proceeding through this route are limited by low carbon efficiencies, in large part due to carbon losses associated with pyruvate decarboxylation to acetyl-CoA. Bypass of pyruvate decarboxylation offers a means to dramatically enhance carbon yields and, in turn, bioprocess economics. Furthermore, we discuss recent advances and prospects for employing the phosphoketolase pathway for direct biosynthesis of acetyl-CoA from carbon substrates, and phosphoketolase-based metabolic engineering strategies for carbon efficient biocatalysis.

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Research Organization:: Johns Hopkins Univ., Baltimore, MD (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: SC0012658

OSTI ID:: 1485594

Alternate ID(s):: OSTI ID: 1250339

Journal Information:: Current Opinion in Biotechnology, Vol. 36, Issue C; ISSN 0958-1669

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 39 works

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Artificial pathway emergence in central metabolism from three recursive phosphoketolase reactions Krüsemann, Jan L.; Lindner, Steffen N.; Dempfle, Marian The FEBS Journal, Vol. 285, Issue 23 https://doi.org/10.1111/febs.14682	journal	November 2018
Holistic bioengineering: rewiring central metabolism for enhanced bioproduction Aslan, Selçuk; Noor, Elad; Bar-Even, Arren Biochemical Journal, Vol. 474, Issue 23 https://doi.org/10.1042/bcj20170377	journal	November 2017
Engineering cytoplasmic acetyl-CoA synthesis decouples lipid production from nitrogen starvation in the oleaginous yeast Rhodosporidium azoricum Donzella, Silvia; Cucchetti, Daniela; Capusoni, Claudia Microbial Cell Factories, Vol. 18, Issue 1 https://doi.org/10.1186/s12934-019-1250-6	journal	November 2019
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Establishing an innovative carbohydrate metabolic pathway for efficient production of 2-keto-l-gulonic acid in Ketogulonicigenium robustum initiated by intronic promoters Wang, Cai-Yun; Li, Ye; Gao, Zi-Wei Microbial Cell Factories, Vol. 17, Issue 1 https://doi.org/10.1186/s12934-018-0932-9	journal	May 2018
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Holistic bioengineering: Rewiring central metabolism for enhanced bioproduction Aslan, Selçuk; Noor, Elad; Bar-Even, Arren ETH Zurich https://doi.org/10.3929/ethz-b-000219514	text	January 2017
Metabolic pathway rewiring in engineered cyanobacteria for solar-to-chemical and solar-to-fuel production from CO₂ Woo, Han Min Bioengineered, Vol. 9, Issue 1 https://doi.org/10.1080/21655979.2017.1317572	journal	May 2017

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