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Title: Energy optimization of a regular macromolecular crystallography beamline for ultra-high-resolution crystallography

In this study, a practical method for operating existing undulator synchrotron beamlines at photon energies considerably higher than their standard operating range is described and applied at beamline 19-ID of the Structural Biology Center at the Advanced Photon Source enabling operation at 30 keV. Adjustments to the undulator spectrum were critical to enhance the 30 keV flux while reducing the lower- and higher-energy harmonic contamination. A Pd-coated mirror and Al attenuators acted as effective low- and high-bandpass filters. The resulting flux at 30 keV, although significantly lower than with X-ray optics designed and optimized for this energy, allowed for accurate data collection on crystals of the small protein crambin to 0.38 Å resolution.
 [1] ;  [2] ;  [3]
  1. Argonne National Lab. (ANL), Argonne, IL (United States); The Univ. of Georgia, Athens, GA (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States); The Univ. of Toledo, Toledo, OH (United States)
Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 1600-5775; JSYRES; PII: S1600577514022619
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Journal of Synchrotron Radiation (Online)
Additional Journal Information:
Journal Name: Journal of Synchrotron Radiation (Online); Journal Volume: 22; Journal Issue: 1; Journal ID: ISSN 1600-5775
International Union of Crystallography
Research Org:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Org:
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION undulator; high energy; monochromator; harmonic