Three dimensional time-gated tracking of non-blinking quantum dots in live cells

Journal Article · Thu Mar 12 00:00:00 EDT 2015 · Proceedings of SPIE - The International Society for Optical Engineering

DOI:https://doi.org/10.1117/12.2082943· OSTI ID:1247683

DeVore, Matthew S. ^[1]; Werner, James H. ^[1]; Goodwin, Peter M. ^[1]; Keller, Aaron M. ^[1]; Hollingsworth, Jennifer A. ^[1]; Wilson, Bridget S. ^[2]; Cleyrat, Cedric ^[2]; Lidke, Diane S. ^[2]; Ghosh, Yagnaseni ^[1]; Stewart, Michael H. ^[3]; Stich, Dominik G. ^[1]; Phipps, Mary E. ^[1]

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1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
2. Univ. of New Mexico, Albuquerque, NM (United States)
3. U.S. Naval Research Lab., Washington, D.C. (United States)

Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. As a result, signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.

• Research Organization: Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE
• Grant/Contract Number: AC52-06NA25396
• OSTI ID: 1247683
• Report Number(s): LA-UR-15-20205
• Journal Information: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 9338; ISSN 0277-786X
• Publisher: SPIE
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
77 NANOSCIENCE AND NANOTECHNOLOGY
Biological Science