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Title: Fine-scale variation in meiotic recombination in Mimulus inferred from population shotgun sequencing

Meiotic recombination rates can vary widely across genomes, with hotspots of intense activity interspersed among cold regions. In yeast, hotspots tend to occur in promoter regions of genes, whereas in humans and mice hotspots are largely defined by binding sites of the PRDM9 protein. To investigate the detailed recombination pattern in a flowering plant we use shotgun resequencing of a wild population of the monkeyflower Mimulus guttatus to precisely locate over 400,000 boundaries of historic crossovers or gene conversion tracts. Their distribution defines some 13,000 hotspots of varying strengths, interspersed with cold regions of undetectably low recombination. Average recombination rates peak near starts of genes and fall off sharply, exhibiting polarity. Within genes, recombination tracts are more likely to terminate in exons than in introns. The general pattern is similar to that observed in yeast, as well as in PRDM9-knockout mice, suggesting that recombination initiation described here in Mimulus may reflect ancient and conserved eukaryotic mechanisms
 [1] ;  [2] ;  [3] ;  [1] ;  [4] ;  [5] ;  [3] ;  [6] ;  [7]
  1. USDOE Joint Genome Inst., Walnut Creek, CA (United States)
  2. Harvard Univ., Cambridge, MA (United States)
  3. USDOE Joint Genome Inst., Walnut Creek, CA (United States); HudsonAlpha Inst. of Biotechnology, Huntsville, AL (United States)
  4. Univ. of Connecticut, Storrs, CT (United States)
  5. Univ. of California, Riverside, CA (United States)
  6. Duke Univ., Durham, NC (United States)
  7. USDOE Joint Genome Inst., Walnut Creek, CA (United States); Univ. of California, Berkeley, CA (United States)
Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 0027-8424
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America; Journal Volume: 110; Journal Issue: 48
National Academy of Sciences, Washington, DC (United States)
Research Org:
Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS Recombination; Play biology; Population genetics