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Title: Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs

We analyse the increasing wealth of metagenomic data collected from diverse environments can lead to the discovery of novel branches on the tree of life. Here we analyse 5.2 Tb of metagenomic data collected globally to discover a novel bacterial phylum (‘Candidatus Kryptonia’) found exclusively in high-temperature pH-neutral geothermal springs. This lineage had remained hidden as a taxonomic ‘blind spot’ because of mismatches in the primers commonly used for ribosomal gene surveys. Genome reconstruction from metagenomic data combined with single-cell genomics results in several high-quality genomes representing four genera from the new phylum. Metabolic reconstruction indicates a heterotrophic lifestyle with conspicuous nutritional deficiencies, suggesting the need for metabolic complementarity with other microbes. Co-occurrence patterns identifies a number of putative partners, including an uncultured Armatimonadetes lineage. The discovery of Kryptonia within previously studied geothermal springs underscores the importance of globally sampled metagenomic data in detection of microbial novelty, and highlights the extraordinary diversity of microbial life still awaiting discovery.
 [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [7] ;  [8] ;  [9] ;  [1] ;  [1] ;  [1] ;  [4] ;  [10] ;  [1] ;  [1] ;  [1]
  1. Department of Energy Joint Genome Institute, Walnut Creek, CA (United States)
  2. Univ. of Calgary, AB (Canada)
  3. Univ. of Nevada, Las Vegas, NV (United States)
  4. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  5. Geological Survey of Canada, Calgary, AB (Canada)
  6. McMaster Univ., Hamilton, ON (Canada)
  7. Miami Univ., Oxford, OH (United States)
  8. Univ. of Alaska-Anchorage, Anchorage, AK (United States)
  9. Sun Yat-Sen University, Guangzhou (China)
  10. Department of Energy Joint Genome Institute, Walnut Creek, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 2041-1723
Grant/Contract Number:
AC52-07NA27344; AC02–05CH11231
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2041-1723
Nature Publishing Group
Research Org:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States